You Searched For: G Biosciences

Catalog Number: (BE-401)

Supplier: G-Biosciences

Description: The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

UOM: 1 * 1 KIT

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Catalog Number: (BE-502)

Supplier: G-Biosciences

Description: Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

UOM: 1 * 1 KIT

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Catalog Number: (786-453)

Supplier: G-Biosciences

Description: The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.

UOM: 1 * 1 KIT

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Catalog Number: (786-488)

Supplier: G-Biosciences

Description: Potassium dihydrogen phosphate 1 mol/l in aqueous solution

UOM: 1 * 1 L

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Catalog Number: (786-651)

Supplier: G-Biosciences

Description: β-Galactosidase assay is for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. It uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic O-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays. This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression. β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570 to 595 nm.

UOM: 1 * 500 Assays

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Catalog Number: (786-627)

Supplier: G-Biosciences

Description: For improved dialysis time and rates.

UOM: 1 * 50 mL

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Supplier: G-Biosciences

Description: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Catalog Number: (786-680)

Supplier: G-Biosciences

Description: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.

UOM: 1 * 50 mL

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Catalog Number: (786-678)

Supplier: G-Biosciences

Description: Swift™ Film Cleaner allows researchers to clean film that has been overexposed or has a high background or speckling without having to repeat experiments. Suitable for all exposed film developed for gel shift assays, Western, Southern and Northern blots.

UOM: 1 * 1 KIT

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Supplier: G-Biosciences

Description: GET™ DNA Template is a spin column format kit suitable for the preparation of DNA templates from blood, cells, fungal and animal tissue samples. The isolated genomic DNA is suitable for PCR and other downstream applications.

Catalog Number: (P317)

Supplier: G-Biosciences

Description: Potassium hydroxide

UOM: 1 * 100 g

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Catalog Number: (P315)

Supplier: G-Biosciences

Description: Potassium chloride

UOM: 1 * 100 g

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Supplier: G-Biosciences

Description: GET™ CLEAN DNA uses high binding affinity GET™ spin columns to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100 bp to 20 kb. GET™ spin columns has an enhanced binding affinity for DNA, thus eliminating loss or damage of DNA.

Supplier: G-Biosciences

Description: EndotoxinOUT™ consists of 6% cross-linked agarose covalently linked to polymyxin B to bind and remove harmful pyrogens from a solution.

Catalog Number: (786-599)

Supplier: G-Biosciences

Description: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.

UOM: 1 * 5 mL

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Catalog Number: (RC-115)

Supplier: G-Biosciences

Description: A colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis.

UOM: 1 * 10 g

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