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Product Image-786-012T

Protein quantitation assay kits, CB™ Protein assay

Supplier: G-Biosciences
Description: CB™ Protein Assay, a Coomassie dye based protein assay based on the Bradford Protein Assay, is suitable for the simple and rapid estimation of protein concentration. The binding of protein to the dye results in a colour change from brown to blue. The change in colour density is proportional to protein concentration. Protein estimation can be performed using as little as 0,5 µg protein.

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Product Image-BE-402B

Experimentation kits, assays for protein quantification

Supplier: G-Biosciences
Description: Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

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Product Image-BE-405

Experimentation kits, enzyme analysis

Catalog Number: (BE-405)
Supplier: G-Biosciences
Description: This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affects the enzyme reaction such as pH and temperature.
UOM: 1 * 1 KIT
Product Image-BE-403

Experimentation kits: biodiversity study and biomass analysis

Catalog Number: (BE-403)
Supplier: G-Biosciences
Description: Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.
UOM: 1 * 1 KIT
Product Image-BE-414

Experimentation kits, size exclusion chromatography

Catalog Number: (BE-414)
Supplier: G-Biosciences
Description: The Size Exclusion Chromatography kit teaches gel filtration or size exclusion chromatography and the use of this method in purification of biological samples. This method is based on separation of protein molecules based on their molecular size. This lab activity involves running size exclusion chromatography for separation of molecules based on their molecular sizes.
UOM: 1 * 1 KIT
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Protease and phosphatase inhibitor, Protease-PhosphataseArrest™

Supplier: G-Biosciences
Description: Protease-PhosphataseArrest™ provides full protection of protein samples from proteases and phosphatases released during the preparation of cell and tissue lysates. Protease-PhosphataseArrest™ contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases along with five phosphatase inhibitors that target all the phosphatase categories: serine/threonine (Ser/Thr) specific, tyrosine specific and dual specificity phosphatases.

Product Image-BE-404

Experimentation kits, hydrophobic and hydrophilic proteins

Catalog Number: (BE-404)
Supplier: G-Biosciences
Description: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.
UOM: 1 * 1 KIT
Product Image-786-440

Peroxide assay kit, PEROXsay™

Supplier: G-Biosciences
Description: PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

Product Image-786-431

SAM Methyltransferase assay, SAMfluoro™

Catalog Number: (786-431)
Supplier: G-Biosciences
Description: The SAMfluoro™ assay is a fluorescent, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
UOM: 1 * 100 Assays
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Mineral oil, white (petroleum)

Catalog Number: (RC-118)
Supplier: G-Biosciences
Description: Mineral oil, white (petroleum)
UOM: 1 * 500 mL
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IPTG (isopropyl-β-D-thiogalactopyranoside) ≥99% Molecular biology grade

Supplier: G-Biosciences
Description: IPTG is a molecular mimic of allolactose that initiates transcription of the lac operon.

Product Image-786-526

Destain solutions

Supplier: G-Biosciences
Description: G-Biosciences' Destain I contains approximately 45% methanol and 9% glacial acetic acid. G-Biosciences' Destain II contains approximately 5% methanol and 7% acetic acid.

Image Unavailable-786-529

Buffer, imidazole buffer solution pH 7,5 (2 mol/L)

Catalog Number: (786-529)
Supplier: G-Biosciences
Description: Imidazole buffer solution pH 7,5 (2 mol/l)
UOM: 1 * 1 L
Product Image-786-576

Ponceau S 0,1% in dil. acetic acid membrane stain

Supplier: G-Biosciences
Description: Ponceau S is a rapid and reversible stain for detecting protein bands on Western blot membranes and can be used with PVDF, nitrocellulose and cellulose acetate membranes*. Ponceau S is a negative stain, which binds to the positively charged amino groups of the protein and it also binds non‐covalently to non‐polar regions in the protein.

Product Image-786-683

Destaining solution, Blue-OUT™ for mass spectrometry

Catalog Number: (786-683)
Supplier: G-Biosciences
Description: Blue-OUT™ for the removal of Coomassie and fluorescent stain prior to mass spectrometry
UOM: 1 * 1 KIT
Image Unavailable-786-670

Accessories for endotoxins and pyrogens removal reagent, EndotoxinOUT™

Supplier: G-Biosciences
Description: Decontamination Solution, Endotoxin free water

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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