Chromatography Products, Solutions & Resources

Liquid Chromatography (HPLC & UHPLC) - Analytical

High-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) are among the most widely used techniques in the chemical and life sciences.

Liquid Chromatography – Sample Preparation

Sample preparation is key but often overlooked step in the chromatography process. Every sample is unique and what works for one preparation may not work as well for another. That’s why Avantor offers a comprehensive range of products.

Gas Chromatography (GC)

Gas chromatography uses helium, hydrogen or other gases to separate, identify, and quantify sample mixtures with reliable results. This technique is widely used in the food, environmental, and chemical industries but also has many other applications.

Thin Layer Chromatography (TLC)

Both thin-layer chromatography and high-performance thin layer chromatography (TLC/HPTLC) are among the most common methods used to separate compounds in a mixture spatially via stationary phase media. 

Mass Spectrometry (MS)

Mass spectrometry is an important tool when specific compounds and materials must be identified with a high degree of precision.

Purification

How do you purify your sample using liquid chromatography? Depending upon the nature of your sample and the purity required, there are several options available ranging from basic flash chromatography to mass directed preparative HPLC.

Column Selection

You need the right HPLC or GC column for your method. There are many options out there. We help you to select the right one for your application.

Bio LC.

You are looking for a solution to analyse or purify a sample. This could be a protein, peptide, characterisation of your mAb or determining antibody/drug ratio (ADR)

Software and Automation

Software is an increasingly important area of analytical chemistry. With software you can automate many operations, improve data quality, reduce errors, improve compliance and increase efficiency.

Services

You need a service to help improve your lab's functioning. From instrument maintenance to bespoke training courses, we could help you.

Featured Products and Services

Unique Stationary Phases

If you are looking for unique stationary phases for complex separations, take a look at these novel stationary phases of the Avantor® ACE® columns range.

Avantor® Hichrom: Experts in Chromatography

If you are looking for expert advice and access to a range of specialist and hard-to-find phases, the experts at Avantor® will help you.

Thermo Scientific Chromatography Columns & Consumables

From sample preparation to sample security and separation, we have the most comprehensive range of chromatography consumables

Pall Acrodisc Premium Syringe Filters

Pall Acrodisc Premium Syringe Filters with universal GHP membrane give you the certainty and assurance you need to ensure trouble-free analysis whatever your sample.

Chromatography resources and solutions

Chromatography is a chemical technique that physically separates a mixture into its component parts. It is a highly effective technique that is used in multiple industries, from food to pharmaceutical, and across all branches of science. Often, it is the only way to separate components, down to the molecular level, from complex mixtures.

Liquid chromatography and gas chromatography are the dominant forms of chromatography. These broadly used techniques include multiple types of chromatography. For instance, high-performance liquid chromatography (HPLC) is a fast, effective process used to maintain product purity and to separate different biological molecules- like proteins and nucleic acids. Affinity chromatography, also a type of liquid chromatography, relies on molecules’ ability to bind together, and then the elution (“washing off”) of non-binding molecules.

Chromatography usually begins with a sample that has individual components that require separation and identification. Components in the sample can be of either solid or liquid origins. A sample mixture can be liquid (for liquid chromatography) or a gas state (for gas chromatography).

There are two phases in chromatography, the mobile phase and the stationary phase. The mobile phase either carries the sample mixture to the stationary phase or disrupts the sample mixture from stationary phase during elution. In liquid chromatography the sample should be compatible with the mobile phase. For gas chromatography the sample, when injected into the process, will convert into a gas—if the sample began in a liquid state.

During the stationary phase the sample mixture moves through a second material, often a solid medium, that differentiates and separates the molecules.

Molecules that share characteristics such as structure or size will group together because they travel through the chromatographic process at the same rate. Charged states of molecules (all matter is either positively, negatively or neutrally charged at the molecular level) in a mixture may cause molecules to behave in a similar like-with-like pattern, or the exact opposite.

At the end of the chromatographic process, the molecules have sorted into distinct, separate, groups.
 

What is chromatography used for?

Chromatography is both broadly used, and highly versatile. It is part of the analysis, production and manufacturing processes in a variety of industries ranging from biopharma and healthcare to food and beverage.

Sometimes the purpose of chromatography is straight forward analyses. Testing food and beverages for quality, and chemical exposure, is very common.

But, because chromatography is used to both quantify and qualify, it has other uses. For instance, it is a well-established technique to analyze small molecule therapeutics and their related substances for characteristics like stability. This allows industries to change formulas, or storage methods, as needed to create better stability or longer shelf-life.

Often, though, the goal of large-scale liquid chromatographic processing is to extract or harvest a specific set of molecules or proteins to use in a biopharmaceutical product- like a therapy or vaccine. In vaccine therapies, chromatography is used at the discovery phase—to find the antibodies most effective at fighting a virus—and then again at the production phase when those antibodies are needed in quantity.

Because chromatography is so widely used, Avantor offers a wide range of highly stable, low-pH, low salt/high salt, equilibration, elution buffers, and buffer components that support chromatographic workflows in multiple industries. Our skilled associates and quality products support multiple types of chromatography techniques at each step of the process, from the bench to the production floor.
 

Avantor is the choice for chromatography

Chromatography can be a complex technique whether you are doing straight forward analysis or large scale biomanufacturing production. Avantor has the resources to make your chromatography projects run efficiently, and effectively. From columns to elution buffers, Avantor has you covered when it comes to selecting the best products and technology for your specific application. We make sourcing simple and support you with our decades of expertise.