You Searched For: 3-(Boc-amino)cyclohexanecarboxylic acid

Description: Catalyses the transfer of the γ-phosphate from ATP to the 5'-hydroxyl group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction).

Catalog Number: EK0031

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: FastRuler ultra low range DNA ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.

Catalog Number: SM1103

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: CCNGGA sites and cuts best at 37 °C in Tango buffer.

Catalog Number: ER1751

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Catalog Number: EP0742

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: RiboRuler™ high range RNA ladder allows precise sizing of RNA molecules in denaturing agarose gels and Northern blotting.

Catalog Number: SM1821

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: GeneJET™ Whole Blood Genomic DNA Purification Mini Kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

Catalog Number: K0782

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: GeneJET™ Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from a wide variety of plant species and tissue types.

Catalog Number: K0802

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Catalog Number: K0241

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: 5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

Catalog Number: K1441

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

Catalog Number: K0692

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

Catalog Number: R0192

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. This nucleotide is used in different molecular biology applications, and it has greater than 99% purity.

Catalog Number: R0441

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Catalog Number: M1682

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: TATG sites and cuts at 37 °C in O buffer (Isoschizomers: FauNDI).

Catalog Number: ER0581

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: HindIII restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions.

Catalog Number: ER0501

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: CCT sites and cuts best at 37 °C in B buffer (Isoschizomers: AatI, PceI, SseBI, StuI).

Catalog Number: ER0421

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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