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Supplier: AGILENT
Description: The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.

Supplier: AGILENT
Description: The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 to 14 kb) or otherwise difficult-to mutagenise plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

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Supplier: AGILENT
Description: The fastest and latest generation of the market-leading QuikChange kits speeds up the protocol for performing single and multiple site-directed mutagenesis to less than 3 hours.

Supplier: AGILENT
Description: These QuikChange II kits provide improved fidelity, while maintaining greater than 80% mutation efficiency for single site mutagenesis.

Supplier: VWR Chemicals
Description: Fast HiFi DNA Polymerase 2 U/µl is a proof reading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning/subcloning, NGS applications and mutagenesis.

SDS

Supplier: VWR Chemicals
Description: Fast HiFi DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning / sub cloning, NGS applications and mutagenesis.

Supplier: AGILENT
Description: The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.

Catalog Number: (F-541)
Supplier: FINNZYMES REAGENTS
Description: Phusion® Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA.
UOM: 1 * 1 KIT


Catalog Number: (GA2100-S)
Supplier: SGI - DNA Synthetic Genomics
Description: The Gibson Assembly® Method is a well-established assembly reaction that can be leveraged to join multiple, mutagenised DNA fragments with overlapping ends. Following mutagenesis, DNA fragments of various lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach lends itself to small and large constructs and encompasses both single and multiple insert assemblies.
UOM: 1 * 5 Tests

SDS


Supplier: Cytiva
Description: High purity dNTPs for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.
Supplier: TriLink BioTechnologies
Description: 2'-Deoxy-P-nucleoside is a degenerate base which behaves as a universal pyrimidine and therefore base pairs with both adenine and guanine. This feature makes it a powerful tool in mutagenesis studies.

New Product

Catalog Number: (BOSSBS-13018R-A350)
Supplier: Bioss
Description: DNA polymerase activity is essential for replication, repair, recombination and mutagenesis. DNA polymerases can often bypass DNA lesions that block DNA replication, thereby allowing the replication of damaged DNA. One such DNA polymerase is the distributive enzyme DNA Pol i, which is encoded by the POLI gene. POLI is located on human chromosome 18q21.2, a region often implicated in the etiology of many human cancers. At thymine templates, DNA Pol i is highly error-prone when replicating undamaged DNA in that it favors the misincorporation of guanine over the correct nucleotide, adenosine. DNA Pol i also promotes the replication of damaged DNA by misincorporating deoxynucleotides opposite DNA lesions. DNA Pol i acts sequentially with DNA Pol Ω, which is essential for damage-induced mutagenesis, to complete the DNA lesion bypass. Therefore, replication involving DNA Pol i is likely to be highly mutagenic.
UOM: 1 * 100 µl


Catalog Number: (BOSSBS-13018R-CY3)
Supplier: Bioss
Description: DNA polymerase activity is essential for replication, repair, recombination and mutagenesis. DNA polymerases can often bypass DNA lesions that block DNA replication, thereby allowing the replication of damaged DNA. One such DNA polymerase is the distributive enzyme DNA Pol i, which is encoded by the POLI gene. POLI is located on human chromosome 18q21.2, a region often implicated in the etiology of many human cancers. At thymine templates, DNA Pol i is highly error-prone when replicating undamaged DNA in that it favors the misincorporation of guanine over the correct nucleotide, adenosine. DNA Pol i also promotes the replication of damaged DNA by misincorporating deoxynucleotides opposite DNA lesions. DNA Pol i acts sequentially with DNA Pol Ω, which is essential for damage-induced mutagenesis, to complete the DNA lesion bypass. Therefore, replication involving DNA Pol i is likely to be highly mutagenic.
UOM: 1 * 100 µl


Catalog Number: (BOSSBS-13018R-FITC)
Supplier: Bioss
Description: DNA polymerase activity is essential for replication, repair, recombination and mutagenesis. DNA polymerases can often bypass DNA lesions that block DNA replication, thereby allowing the replication of damaged DNA. One such DNA polymerase is the distributive enzyme DNA Pol i, which is encoded by the POLI gene. POLI is located on human chromosome 18q21.2, a region often implicated in the etiology of many human cancers. At thymine templates, DNA Pol i is highly error-prone when replicating undamaged DNA in that it favors the misincorporation of guanine over the correct nucleotide, adenosine. DNA Pol i also promotes the replication of damaged DNA by misincorporating deoxynucleotides opposite DNA lesions. DNA Pol i acts sequentially with DNA Pol Ω, which is essential for damage-induced mutagenesis, to complete the DNA lesion bypass. Therefore, replication involving DNA Pol i is likely to be highly mutagenic.
UOM: 1 * 100 µl


Catalog Number: (BOSSBS-13018R-A555)
Supplier: Bioss
Description: DNA polymerase activity is essential for replication, repair, recombination and mutagenesis. DNA polymerases can often bypass DNA lesions that block DNA replication, thereby allowing the replication of damaged DNA. One such DNA polymerase is the distributive enzyme DNA Pol i, which is encoded by the POLI gene. POLI is located on human chromosome 18q21.2, a region often implicated in the etiology of many human cancers. At thymine templates, DNA Pol i is highly error-prone when replicating undamaged DNA in that it favors the misincorporation of guanine over the correct nucleotide, adenosine. DNA Pol i also promotes the replication of damaged DNA by misincorporating deoxynucleotides opposite DNA lesions. DNA Pol i acts sequentially with DNA Pol Ω, which is essential for damage-induced mutagenesis, to complete the DNA lesion bypass. Therefore, replication involving DNA Pol i is likely to be highly mutagenic.
UOM: 1 * 100 µl


Catalog Number: (BOSSBS-6383R-FITC)
Supplier: Bioss
Description: The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization.
UOM: 1 * 100 µl


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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