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You Searched For: Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.


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Cell lysis buffers

Catalog Number: (ROCKMB-078-0015)
Supplier: Rockland Immunochemicals
Description: Cell lysis buffer is used to lyse cells under non-denaturing conditions.
UOM: 1 * 15 mL
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Lysis buffer, pH 8,0, for isolation of His-tag modified proteins

Catalog Number: (J63892.AP)
Supplier: Thermo Fisher Scientific
Description: Lysis buffer, for isolation of His-tag modified proteins, pH value: 8,0, Thermo Fisher Scientific
UOM: 1 * 500 mL

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RBC lysis buffer for human

Supplier: Thermo Fisher Scientific
Description: RBC lysis buffer for human, Thermo Fisher Scientific

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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of <i>E. coli</i>, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

Product Image-PIER78510

T-PER™ Tissue Protein Extraction Reagent

Catalog Number: (PIER78510)
Supplier: Thermo Fisher Scientific
Description: Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.
UOM: 1 * 500 mL

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MNE buffer, pH 6,5

Supplier: Thermo Fisher Scientific
Description: MNE buffer, pH 6.5, Thermo Fisher Scientific

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NP-40 lysis buffer, low salt

Supplier: Thermo Fisher Scientific
Description: NP-40 lysis buffer, low salt, Thermo Fisher Scientific

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GTE buffer

Supplier: Thermo Fisher Scientific
Description: Thermo Fisher Scientific

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HEPES lysis buffer with NP-40 (2X)

Catalog Number: (J63867.AK)
Supplier: Thermo Fisher Scientific
Description: HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
UOM: 1 * 250 mL

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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RIPA Lysis and Extraction Buffer

Supplier: G-Biosciences
Description: A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.

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E. coli lysis buffer

Catalog Number: (J63402.AK)
Supplier: Thermo Fisher Scientific
Description: E. coli lysis buffer, Thermo Fisher Scientific
UOM: 1 * 250 mL

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Protein extraction reagent, BugBuster® 10X, Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

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PIPES lysis buffer with NP-40 (2X)

Catalog Number: (J60568.AK)
Supplier: Thermo Fisher Scientific
Description: PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
UOM: 1 * 250 mL

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NP-40 lysis buffer (2X)

Catalog Number: (J62805.AK)
Supplier: Thermo Fisher Scientific
Description: NP-40 lysis buffer (2X), Thermo Fisher Scientific
UOM: 1 * 250 mL

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HEPES protein extraction buffer (5X)

Catalog Number: (J63297.AK)
Supplier: Thermo Fisher Scientific
Description: Thermo Fisher Scientific
UOM: 1 * 250 mL

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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