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You Searched For: Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.


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Product Image-786-176

Protein extraction kit, bacterial PE LB™

Supplier: G-Biosciences
Description: Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Product Image-786-180

Protein extraction kit, mammalian cell PE LB™

Catalog Number: (786-180)
Supplier: G-Biosciences
Description: Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
UOM: 1 * 500 mL

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Product Image-PIER17908

Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer

Supplier: Thermo Fisher Scientific
Description: Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.

Product Image-786-178

Protein extraction kit, yeast PE LB™

Supplier: G-Biosciences
Description: Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.

Product Image-A4150.0500

Lysis buffer, CTAB

Supplier: PanReac AppliChem
Description: Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.

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Product Image-PIER89901

RIPA lysis and extraction buffer, Pierce™

Supplier: Thermo Fisher Scientific
Description: The RIPA lysis and extraction buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.

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Image Unavailable-OMEGRBB-4000

RBB Buffers

Supplier: OMEGA BIO-TEK
Description: RBB Buffer, 1000 ml, Omega Bio-Tek

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Product Image-PIER16189

Luciferase cell lysis buffer (2X), Pierce™

Catalog Number: (PIER16189)
Supplier: Thermo Fisher Scientific
Description: The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.
UOM: 1 * 250 mL
Product Image-786-PSB

Protein solubilisation buffer

Catalog Number: (786-PSB)
Supplier: G-Biosciences
Description: FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.
UOM: 1 * 50 mL
Product Image-PIER89802

I-PER™ Insect Cell Protein Extraction Reagents

Catalog Number: (PIER89802)
Supplier: Thermo Fisher Scientific
Description: The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.
UOM: 1 * 250 mL
Product Image-PIER87788

IP lysis buffer, Pierce™

Supplier: Thermo Fisher Scientific
Description: The IP lysis buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS.

Image Unavailable-PDR045

DNA/RNA clean-up buffers

Supplier: OMEGA BIO-TEK
Description: Binding buffer XP2, Omega Bio-Tek

Product Image-AATB20012

ReadiUse™ Mammalian Cell Lysis Buffer *5X*

Catalog Number: (AATB20012)
Supplier: AAT BIOQUEST
Description: All our ReadiUse™ reagents require minimal hands-on time.
UOM: 1 * 10 mL
Product Image-PIER78999

Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Catalog Number: (PIER78999)
Supplier: Thermo Fisher Scientific
Description: This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.
UOM: 1 * 500 mL
Product Image-AATB24100

ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)

Catalog Number: (AATB24100)
Supplier: AAT BIOQUEST
Description: Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.
UOM: 1 * 10 mL
Product Image-AATB24102

ReadiUse™ Yeast Cell Lysis Buffer, 5X

Catalog Number: (AATB24102)
Supplier: AAT BIOQUEST
Description: ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.
UOM: 1 * 10 mL

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