Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®
Supplier: Merck Millipore (Novagen)Total Ratings: 0
Avg. Ratings: 0.0 out of 5
BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.
The standard BugBuster® reagent is supplied as a Tris-buffered, 1X ready-to-use liquid that is stable at room temperature. The 500 ml size is also available bundled with 10000 U Benzonase® Nuclease (provided in a separate vial) for the preparation of low viscosity extracts and/or removal of nucleic acids from protein preparations.
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