You Searched For: STEMCELL Technologies

Catalog Number: (STMC01700)

Supplier: STEMCELL Technologies

Description: ALDEFLUOR™ kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH - 1 kit.

UOM: 1 * 1 KIT

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Supplier: STEMCELL Technologies

Description: Immunomagnetic isolation of untouched human B cells.

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Catalog Number: (STMC18001)

Supplier: STEMCELL Technologies

Description: The EasySep™ 'The Big Easy' magnet is designed for cell separation procedures using EasySep™ reagents when processing a large number of cells, or when using RoboSep™.

UOM: 1 * 1 items

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Catalog Number: (STMC03805)

Supplier: STEMCELL Technologies

Description: Hybridoma growth medium with hypoxanthine and thymidine (serum-containing).

UOM: 1 * 500 mL

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Catalog Number: (STMC60082)

Supplier: STEMCELL Technologies

Description: Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD5, unconjugated.

UOM: 1 * 100 µG

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Supplier: STEMCELL Technologies

Description: Immunodensity depletion of granulocytes.

Catalog Number: (STMC60074AZ)

Supplier: STEMCELL Technologies

Description: Rat monoclonal IgM, kappa isotype control antibody, APC-conjugated.

UOM: 1 * 100 µG

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Supplier: STEMCELL Technologies

Description: Rat monoclonal IgG1 antibody against human, mouse, dog E-cadherin (CD324), unconjugated.

Catalog Number: (STMC05752)

Supplier: STEMCELL Technologies

Description: Medium for differentiation of human neural stem and progenitor cells.

UOM: 1 * 1 KIT

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Supplier: STEMCELL Technologies

Description: Interleukin 10 (IL-10) is the founding member of the IL-10 family of class II cytokines. All of the IL-10 cytokine family members have a four-helix bundle consisting of α-helical folds. Upon binding to its receptor, IL-10 activates signaling through JAK1 and STAT3. It is produced by dendritic cells, macrophages, and CD4+ T cells, as well as mast cells, NK cells, neutrophils, and B cells, under specific stimulating conditions (Saraiva and O'Garra). IL-10 can inhibit the expression of pro-inflammatory cytokines and promote healing processes, and is important for the function of regulatory T cells. IL-10 also enhances B cell proliferation, immunoglobulin secretion, and class II MHC expression, while IL-10 produced by macrophages inhibits activation of neighboring macrophages, thus allowing a level of self-regulation (Ouyang <i>et al.</i>).

Supplier: STEMCELL Technologies

Description: Thrombopoietin (TPO) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and <i>in vivo</i>. TPO stimulates the proliferation and maturation of megakaryocytes and has an important role in regulating the level of circulating platelets <i>in vivo</i>. TPO also promotes the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multilineage progenitor cells. It is commonly used with other cytokines such as stem cell factor (SCF) and Flt3/Flk-2 Ligand to promote expansion of primitive hematopoietic cells in culture (Hitchcock andamp; Kaushansky). The TPO receptor, c-Mpl, is expressed at all stages of megakaryopoiesis, from hematopoietic stem and progenitor cells to mature platelets.

Supplier: STEMCELL Technologies

Description: Interleukin 13 (IL-13) is a type I cytokine, and signals through type I cytokine receptors to activate JAK/STAT and IRS-1/IRS-2 pathways. IL-13 is produced by T cells and innate lymphoid cells (Pulendran and Artis) and it inhibits production of pro-inflammatory cytokines, prostaglandins, and reactive oxygen and nitrogen species by monocytes and macrophages (Hershey). Unlike human B cells, IL-13 has no effect on mouse B cell development and function. IL-13 promotes eosinophil survival, activation, and recruitment, and also activates mast cells (Hershey). IL-13 regulates gastrointestinal parasite expulsion, airway hyperresponsiveness, allergic inflammation, tissue eosinophilia, goblet cell hyperplasia, intracellular parasitism, tissue remodeling, tumor cell growth, and fibrosis. IL-13 induces hypercontractility of smooth muscles by directly acting on the muscle cells as well as the enteric nerves (Wynn).

Supplier: STEMCELL Technologies

Description: Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Yang <i>et al.</i>; Dorssers <i>et al.</i>; Broughton <i>et al.</i>). IL-3 is produced by activated T cells and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by basophils and eosinophils (Broughton <i>et al.</i>). The IL-3 receptor consists of a unique alpha subunit (CD123) and a beta common subunit (βc or CD131) that is shared with the receptors for IL-5 and GM-CSF, and is the principal signal transduction subunit for these cytokines. IL-3 binding to the heterodimeric receptor activates JAK/STAT, MAPK, and PI3K signaling pathways (Woodcock <i>et al.</i>).

Supplier: STEMCELL Technologies

Description: Sonic hedgehog (Shh) is a member of the Hedgehog family of secreted signaling proteins that has a prominent role in patterning during early mammalian development (Ho and Scott). It is expressed during early embryogenesis in a variety of tissues (Finco <i>et al.</i>). During patterning of the central nervous system, Shh directly acts on cells to specify neural cell fates, regulates proliferation and survival of oligodendrocytes precursors and neural crest cells and stimulates proliferation, differentiation and axon growth (Ho and Scott; Martí). Shh also regulates T cell differentiation and activation (Crompton <i>et al.</i>). Recombinant human Shh (C24II; rhShh) is a fully biologically active molecule.

Supplier: STEMCELL Technologies

Description: Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf and Nicola). G-CSF causes a range of effects including a transient reduction of SDF-1 expression (Petit <i>et al.</i>), the activation of metalloproteases that cleave VCAM-1 (Levesque <i>et al.</i>), and the release of norepinephrine from the sympathetic nervous system (Katayama <i>et al.</i>), leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. Mouse G-CSF was first purified from cultures of the WEHI-3B myelomonocytic leukemia cell line as the inducer of the terminal differentiation of WEHI-3B and other myeloid leukemia cell lines (Nicola <i>et al.</i>). It was later cloned in monkey COS cells from a cDNA library prepared with mRNA derived from mouse fibrosarcoma NFSA cells that produce G-CSF constitutively (Tsuchiya <i>et al.</i>). Binding of G-CSF to its receptor leads to activation of the JAK/STAT, MAPK, PI3K, and AKT signal transduction pathways.

Supplier: STEMCELL Technologies

Description: Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine that activates NF-kB, MAPK, and PI3K/AKT pathways. Activated T cells and macrophages are the primary producers of TNF-α in response to inflammation and infectious conditions. Many other cell types have been shown to produce TNF-α, among them B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts. TNF-α has cytotoxic effects on cancer cells in vitro by stimulating anti-tumor immuno- suppressive responses. TNF-α stimulates expression of E- and P-selectins, thus facilitating adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells (Zelová and Hosek). Other effects of TNF-α include vasodilatation and edema formation.