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Product Image-SM1211

DNA ladders, GeneRuler™ and O'GeneRuler™

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific™ GeneRuler™ and O'GeneRuler™ DNA ladders are mixtures of chromatography-purified individual DNA fragments. The GeneRuler™ line includes the most popular ladders, such as the 100 bp and 1 kb DNA Ladder. GeneRuler™ DNA ladders are available in two formats: Conventional (provided in TE buffer) and a ready-to-use format (premixed with 6X DNA Loading Dye which contains bromophenol blue and xylene cyanol FF). O'GeneRuler™ DNA ladders are another ready-to-use version of GeneRuler™ DNA ladders that are premixed with 6X Orange DNA Loading Dye.

Product Image-K1232

PCR cloning kit, CloneJET

Supplier: Thermo Fisher Scientific
Description: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Product Image-FD1464

Restriction enzymes, AgeI, FastDigest™, Fermentas

Catalog Number: (FD1464)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
UOM: 1 * 1 KIT
Product Image-ER1921

Restriction enzymes, EcoRII, 10 U/µl, Fermentas

Catalog Number: (ER1921)
Supplier: Thermo Fisher Scientific
Description: CCWGG sites and cuts best at 37 °C in O buffer.
UOM: 1 * 1 KIT
Product Image-ER0053

Restriction enzymes, BamHI, HC, Fermentas

Catalog Number: (ER0053)
Supplier: Thermo Fisher Scientific
Description: GATCC sites and cuts best at 37 °C in its own unique buffer.
UOM: 1 * 1 KIT
Product Image-ER0303

Restriction enzymes, Eco32I (EcoRV) (HC), Fermentas

Catalog Number: (ER0303)
Supplier: Thermo Fisher Scientific
Description: ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.
UOM: 1 * 1 KIT
Product Image-R0812

5-Fluoroorotic acid enzyme substrate

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase

Image Unavailable-R0862

Dithiothreitol (DTT, Cleland's reagent)

Catalog Number: (R0862)
Supplier: Thermo Fisher Scientific
Description: Dithiothreitol (DTT, Cleland's reagent)
UOM: 1 * 1 KIT
Product Image-EN0581

Exonuclease I

Supplier: Thermo Fisher Scientific
Description: Exonuclease I (ExoI) degrades single-stranded DNA in a 3' to 5'direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

Product Image-EO0461

Agarase

Catalog Number: (EO0461)
Supplier: Thermo Fisher Scientific
Description: Agarase is an enzyme that specifically digests the agarose polysaccharide core into neoagaro-oligosaccharides.
UOM: 1 * 1 KIT

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Product Image-EM0821

CpG methyltransferase

Catalog Number: (EM0821)
Supplier: Thermo Fisher Scientific
Description: CpG methyltransferase enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5'-CpG-3' context. Thermo Scientific M.SssI is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37 °C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.
UOM: 1 * 1 KIT
Product Image-SO132

Oligo(dT)₁₈ primer

Supplier: Thermo Fisher Scientific
Description: Oligo(dT)₁₈ primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready to use, 20X concentrated aqueous solution. Oligo(dT)₁₈ primer has been functionally tested in first strand cDNA synthesis.

Product Image-K9021

Master mixes, DreamTaq™ Hot Start PCR master mix (2X)

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready to use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq™ buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. The green master mix includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

Product Image-FD0663

Restriction enzymes, SmaI, FastDigest™, Fermentas

Catalog Number: (FD0663)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
UOM: 1 * 1 KIT
Product Image-ER0302

Restriction enzymes, Eco32I (EcoRV), Fermentas

Supplier: Thermo Fisher Scientific
Description: ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.

Image Unavailable-EP0282

Taq DNA polymerase (native, without BSA)

Catalog Number: (EP0282)
Supplier: Thermo Fisher Scientific
Description: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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