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Catalog Number: (1B1409-100RXN)
Supplier: VWR Chemicals
Description: Hot Start PCR-To-Gel Taq Master Mix, 2X is a ready to use reaction cocktail that contains all required components, except primers and template DNA, for routine PCR amplification of DNA fragments up to 4 kb.
UOM: 1 * 1 KIT

SDS


Supplier: Biotium
Description: TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.

Supplier: Thermo Fisher Scientific
Description: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

Supplier: AGILENT
Description: Herculase II Fusion DNA Polymerases produce superior yields with extension times as short as 15 s/kb, even with genomic DNA targets up to 23 kb.

Supplier: AGILENT
Description: PfuUltra High-Fidelity DNA Polymerase AD features a reformulated buffer system for increased economy with the same high performance as the original PfuUltra DNA Polymerase for robust, ultra-high-fidelity PCR.

Supplier: AGILENT
Description: Detects low copy number target and withstands PCR inhibitors without extensive purification.

Catalog Number: (ENZOBMLAK5160001)
Supplier: ENZO LIFE SCIENCES
Description: A Fluor-de-Lys® fluorescent assay system. The HDAC6 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC6 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC6 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.
UOM: 1 * 1 KIT


Catalog Number: (SOLUK-7413-25)
Supplier: TriLink BioTechnologies
Description: <p><span style="color:black">TriLink’s CleanCap® AG (3′ OMe) CleanScript™ IVT kit provides essential components for mRNA synthesis using co-transcriptional capping by in vitro transcription (IVT). The kit components are sufficient to run 25 of 100 µl reactions following our recommended IVT protocol with co-transcriptional capping. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes including CleanScribe RNA polymerase, 10X CleanScript IVT reaction buffer, and a linearized FLuc DNA control template.</span></p>
UOM: 1 * 100 µl

New Product


Catalog Number: (AVIVOKEH00344)
Supplier: Aviva Systems Biology
Description: Aviva Systems Biology’s B2m ELISA kit (Mouse) (OKEH00344) is based on standard sandwich enzyme-linked immunosorbent assay technology. An antibody specific for B2m has been pre-coated onto a 96-well plate (12×8 well strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for B2m is added, incubated and followed by washing. Avidin-Peroxidase conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalysed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample B2m captured in the well.
UOM: 1 * 1 KIT


Catalog Number: (AVIVOKEH03665)
Supplier: Aviva Systems Biology
Description: Aviva Systems Biology RHOT1 ELISA Kit (Human) (OKEH03665) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RHOT1 has been pre-coated onto a 96-well plate (12×8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for RHOT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RHOT1 captured in well.
UOM: 1 * 1 KIT


Catalog Number: (AVIVOKWB00278)
Supplier: Aviva Systems Biology
Description: Aviva Systems Biology Serpina3c ELISA Kit (Mouse) (OKWB00278) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpina3c has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpina3c is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpina3c captured in the well.
UOM: 1 * 1 KIT


Supplier: VWR Chemicals
Description: The fast extract DNA solution provides rapid and efficient extraction of PCR-ready DNA from mammalian and plant tissues. DNA is ready in eight minutes. The one-reagent protocol is divided into two simple heating steps, which can be directly followed by PCR analysis, such as screening and genotyping.

Catalog Number: (786-258)
Supplier: G-Biosciences
Description: Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 KIT


Catalog Number: (ENZOBMLAK5180001)
Supplier: ENZO LIFE SCIENCES
Description: A Fluor-de-Lys® fluorescent assay system. The HDAC8 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC8 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC8 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.
UOM: 1 * 1 KIT


Supplier: Corning
Description: The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Catalog Number: (AVIVOKEH03072)
Supplier: Aviva Systems Biology
Description: Aviva Systems Biology APOC1 ELISA Kit (Mouse) (OKEH03072) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apoc1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Apoc1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apoc1 captured in well.
UOM: 1 * 1 KIT


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
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This product has been blocked by your organisation. Please contact your purchasing department for more information.
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Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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