Mouse SERPINA3C ELISA Kit

Supplier: Aviva Systems Biology
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OKWB00278
AVIVOKWB00278EA 608 GBP
AVIVOKWB00278
Mouse SERPINA3C ELISA Kit
Assays ELISAs

Aviva Systems Biology Serpina3c ELISA Kit (Mouse) (OKWB00278) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpina3c has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpina3c is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpina3c captured in the well.


Mean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%

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SPECIFICATIONS

Environmentally Preferable
Target protein
Primary antibody reactivity
Assay Type Sandwich
Assay Principle Quantitative
Host Mouse
Conjugate ELISA HRP
Sensitivity 46.9 pg/ml
Detection method Colorimetric, OD 450 nm
Specificity
Sample type Serum, plasma, tissue homogenates, cell cultures, other biological fluids
Storage conditions

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