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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®

Supplier: Merck Millipore (Novagen)
Description: BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of <i>E. coli</i>, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

Product Image-28-9435-22

2-D Protein Extraction Buffer

Catalog Number: (28-9435-22)
Supplier: Cytiva
Description: Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.
UOM: 1 * 1 KIT
Product Image-PIER90402

Mass tagging, mass tag labelling reagents, AminoxyTMT™, Pierce™

Supplier: Thermo Fisher Scientific
Description: The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) Label Reagents enable multiplexed characterisation and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidised proteins) by mass spectrometry (MS).

Product Image-PIER90114

Accessories for Tandem Mass Tag™ reagents and experiments

Supplier: Thermo Fisher Scientific
Description: Immobilised Anti-TMT™ antibody resin

Product Image-786-428

Adenine deaminase

Catalog Number: (786-428)
Supplier: G-Biosciences
Description: Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.
UOM: 1 * 0,25 mL
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CHAPS lysis buffer

Supplier: Thermo Fisher Scientific
Description: For cell lysis and protein extraction.

SDS Certificates

Product Image-PIER89842

Membrane protein extraction kit, Pierce™ Mem-PER™ Plus

Catalog Number: (PIER89842)
Supplier: Thermo Fisher Scientific
Description: The Mem-PER™ Plus Membrane Protein Extraction Kit enables small-scale solubilisation and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure. It effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure.
UOM: 1 * 1 KIT

SDS


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HEPES protein extraction buffer (5X)

Catalog Number: (J63297.AK)
Supplier: Thermo Fisher Scientific
Description: Thermo Fisher Scientific
UOM: 1 * 250 mL

SDS Certificates


Product Image-78833.

Nuclear and cytoplasmic extraction reagents, NE-PER

Supplier: Thermo Fisher Scientific
Description: The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Product Image-PIER78115

Inclusion body solubilisation reagent, Pierce™

Catalog Number: (PIER78115)
Supplier: Thermo Fisher Scientific
Description: Inclusion Body Solubilisation Reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents.
UOM: 1 * 100 mL
Product Image-PIER28340

Detergent sampler Kit, Surfact-Amps™

Catalog Number: (PIER28340)
Supplier: Thermo Fisher Scientific
Description: Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.
UOM: 1 * 1 KIT
Product Image-PIER87779

Pierce™ Detergent Removal Spin Columns and Resin

Supplier: Thermo Fisher Scientific
Description: Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.

Product Image-PIER20230

Immobilised trypsin, TPCK treated, Pierce™

Catalog Number: (PIER20230)
Supplier: Thermo Fisher Scientific
Description: Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.
UOM: 1 * 2 mL
Product Image-PIER85115

Detergent solutions, Surfact-Amps™

Supplier: Thermo Fisher Scientific
Description: Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.

Product Image-PIER90305

Trypsin protease, MS grade

Supplier: Thermo Fisher Scientific
Description: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

Product Image-786-232

FOCUS™ Protein Alkylation Kit

Catalog Number: (786-232)
Supplier: G-Biosciences
Description: The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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