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Product Image-28-9435-22

2-D Protein Extraction Buffer

Catalog Number: (28-9435-22)
Supplier: Cytiva
Description: Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.
UOM: 1 * 1 KIT
Product Image-PIER90003

Phosphoprotein enrichment kit, Pierce™

Catalog Number: (PIER90003)
Supplier: Thermo Fisher Scientific
Description: Pierce™ Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.
UOM: 1 * 10 items
Product Image-786-248

Protein fractionation kit, FOCUS™ cytoplasmic and nuclear proteins

Catalog Number: (786-248)
Supplier: G-Biosciences
Description: FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit for the fractionation of nuclear and cytoplasmic proteins from cells and tissues.
UOM: 1 * 1 KIT
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Bacterial DNA kit, E.Z.N.A.®

Supplier: OMEGA BIO-TEK
Description: The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

SDS

Product Image-PIER89895

Pierce™ In-solution Tryptic Digestion and Guanidination Kit

Catalog Number: (PIER89895)
Supplier: Thermo Fisher Scientific
Description: The In-Solution Tryptic Digestion and Guanidination Kit contains proteomics grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.
UOM: 1 * 1 KIT
Product Image-PIER89899

Ubiquitin enrichment kit, Pierce™

Catalog Number: (PIER89899)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Ubiquitin enrichment kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.
UOM: 1 * 1 KIT
Product Image-786-232

FOCUS™ Protein Alkylation Kit

Catalog Number: (786-232)
Supplier: G-Biosciences
Description: The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.
UOM: 1 * 1 KIT
Product Image-REST25849

QuEChERS extraction salts, Q-sep®

Supplier: Restek
Description: QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.

Product Image-PIER89881

Cell surface protein isolation kit, Pierce™

Catalog Number: (PIER89881)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.
UOM: 1 * 1 KIT
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Protein extraction kits, BugBuster® Plus Lysonase™

Supplier: Merck Millipore (Novagen)
Description: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

Product Image-PIER89871

In-Gel tryptic digestion kit, Pierce™

Catalog Number: (PIER89871)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ In-Gel Tryptic Digestion Kit provides a complete set of reagents for digesting and recovering peptide fragments from approximately 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).
UOM: 1 * 1 KIT
Product Image-786-179

Protein extraction kit, yeast PE LB™

Supplier: G-Biosciences
Description: Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.

Product Image-786-125

Protein concentration kit, OrgoSol-PROTEIN-Concentrate™

Catalog Number: (786-125)
Supplier: G-Biosciences
Description: The OrgoSol-PROTEIN-Concentration™ Kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains the biological activity of most proteins.
UOM: 1 * 50 mL
Product Image-PIER88210

Accessories for SILAC protein quantitation kit, Pierce™

Supplier: Thermo Fisher Scientific
Description: L-Leucine, (¹³C₆), Pierce™

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Thrombin cleavage capture kit, Millipore®

Catalog Number: (69022-3)
Supplier: Merck Millipore (Novagen)
Description: The thrombin cleavage capture kit is designed for cleavage of fusion proteins followed by convenient and quantitative removal of thrombin protease. The method is based on the use of biotinylated thrombin for digestion and its subsequent removal with streptavidin agarose.
UOM: 1 * 1 KIT
Product Image-PIER89875

Albumin/IgG removal kit, Pierce™

Catalog Number: (PIER89875)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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