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Product Image-786-230

FOCUS™ protein reductant kit

Catalog Number: (786-230)
Supplier: G-Biosciences
Description: FOCUS™ Protein Reductant Kit is a water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.
UOM: 1 * 1 KIT
Product Image-786-247

Protein fractionation kit, FOCUS™ soluble and insoluble

Catalog Number: (786-247)
Supplier: G-Biosciences
Description: FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.
UOM: 1 * 1 KIT
Product Image-786-232

FOCUS™ Protein Alkylation Kit

Catalog Number: (786-232)
Supplier: G-Biosciences
Description: The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.
UOM: 1 * 1 KIT
Product Image-786-253

Protein fractionation kit, FOCUS™ Glycoprotein

Catalog Number: (786-253)
Supplier: G-Biosciences
Description: FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.
UOM: 1 * 1 KIT
Product Image-786-231

FOCUS™ protein reduction-alkylation kit

Catalog Number: (786-231)
Supplier: G-Biosciences
Description: The FOCUS™ Reduction-Alkylation Kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.
UOM: 1 * 1 KIT
Product Image-786-273

Antibody fab fragmentation kit

Supplier: G-Biosciences
Description: Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.

Product Image-786-260

FOCUS™ SubCell, protein fractionation kit

Catalog Number: (786-260)
Supplier: G-Biosciences
Description: FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.
UOM: 1 * 1 KIT
Product Image-786-126

Protein concentration kit, Column-PROTEIN-Concentrate™

Catalog Number: (786-126)
Supplier: G-Biosciences
Description: The Column-PROTEIN-Concentrate™ Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilises any protein in a low salt buffer between pH 2 to 12. The binding capacity is ~0,5 mg protein/ml Protein Binding Resin.
UOM: 1 * 1 KIT

SDS


Product Image-PIER90065

Isobaric mass tagging kits and reagent sets, TMT™

Supplier: Thermo Fisher Scientific
Description: Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry. The Tandem Mass Tag (TMT) reagent family consists of TMTduplex and TMTsixplex sets which are specially designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation.

Product Image-786-125

Protein concentration kit, OrgoSol-PROTEIN-Concentrate™

Catalog Number: (786-125)
Supplier: G-Biosciences
Description: The OrgoSol-PROTEIN-Concentration™ Kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains the biological activity of most proteins.
UOM: 1 * 50 mL
Product Image-786-018

Protein fractionation kit, FOCUS™ Global Fractionation

Catalog Number: (786-018)
Supplier: G-Biosciences
Description: FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.
UOM: 1 * 1 KIT
Product Image-786-277

Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits

Supplier: G-Biosciences
Description: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Product Image-786-257

FOCUS™ Yeast proteome kits

Catalog Number: (786-257)
Supplier: G-Biosciences
Description: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 KIT
Product Image-PIER88279

Mouse embryonic fibroblast isolation kit, Pierce™

Catalog Number: (PIER88279)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.
UOM: 1 * 1 KIT
Product Image-786-275

Antibody F(ab)₂ fragmentation kits

Supplier: G-Biosciences
Description: The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

Product Image-786-256

Protein enrichment kit, FOCUS™ PhosphoRich™

Supplier: G-Biosciences
Description: FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20 mg phosphorylated ovalbumin per column. The resin columns supplied with the kit can be reused, if regenerated and stored properly. FOCUS™ PhosphoRich™ is suitable for 2D gel electrophoresis and for proteomics and cell signalling studies.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
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Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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