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Product Image-786-257

FOCUS™ Yeast proteome kits

Catalog Number: (786-257)
Supplier: G-Biosciences
Description: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 KIT
Product Image-786-018

Protein fractionation kit, FOCUS™ Global Fractionation

Catalog Number: (786-018)
Supplier: G-Biosciences
Description: FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.
UOM: 1 * 1 KIT
Product Image-786-246

Proteome kits, mammalian proteome, FOCUS™

Catalog Number: (786-246)
Supplier: G-Biosciences
Description: Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.
UOM: 1 * 1 KIT
Product Image-786-256

Protein enrichment kit, FOCUS™ PhosphoRich™

Supplier: G-Biosciences
Description: FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20 mg phosphorylated ovalbumin per column. The resin columns supplied with the kit can be reused, if regenerated and stored properly. FOCUS™ PhosphoRich™ is suitable for 2D gel electrophoresis and for proteomics and cell signalling studies.

Product Image-PIER88289

Accessories for primary cell isolation kits, Pierce™

Supplier: Thermo Fisher Scientific
Description: Neuronal culture medium, Pierce

Image Unavailable-ENZOBMLPW1075A0001

Proteasome Purification Kit

Catalog Number: (ENZOBMLPW1075A0001)
Supplier: ENZO LIFE SCIENCES
Description: Proteasome Purification Kit is a high integrity kit for the isolation and purification of proteasome complexes from biological samples such as cell lysates, tissue extracts, plasma, serum, confirmed by Western blot analysis.
UOM: 1 * 10 Tests
Product Image-PIER88279

Mouse embryonic fibroblast isolation kit, Pierce™

Catalog Number: (PIER88279)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.
UOM: 1 * 1 KIT
Product Image-786-259

Proteome kits, FOCUS™ Plant Proteome

Catalog Number: (786-259)
Supplier: G-Biosciences
Description: Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.
UOM: 1 * 1 KIT
Product Image-786-273

Antibody fab fragmentation kit

Supplier: G-Biosciences
Description: Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.

Product Image-786-022

Protein fractionation kit, FOCUS™ mitochondria

Catalog Number: (786-022)
Supplier: G-Biosciences
Description: FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.
UOM: 1 * 1 KIT
Product Image-786-258

Proteome kits, FOCUS™ Bacterial Proteome

Catalog Number: (786-258)
Supplier: G-Biosciences
Description: Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM: 1 * 1 KIT
Product Image-786-251

Albumin removal kit, AlbuminOUT™

Supplier: G-Biosciences
Description: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Image Unavailable-ENZOADI900199

[pSer326]HSF1 ELISA Kits

Catalog Number: (ENZOADI900199)
Supplier: ENZO LIFE SCIENCES
Description: The HSF1 EIA kit is a complete kit for the quantitative determination of HSF1 phosphorylated at Ser326 in cell lysates and tissue extracts from human and mouse origins. This is a sensitive ELISA kit for the key transcription factor for heat shock protein (chaperone) research.
UOM: 1 * 1 KIT
Image Unavailable-ENZOADI900198

HSF1 ELISA Kits

Catalog Number: (ENZOADI900198)
Supplier: ENZO LIFE SCIENCES
Description: The HSF1 EIA kit is a complete kit for the quantitative determination of HSF1 in cell lysates and tissue extracts from human and mouse origins. This is a Sensitive ELISA kit for this key transcription factor for heat shock protein (chaperone) research.
UOM: 1 * 1 KIT
Product Image-786-250

Protein fractionation kit, FOCUS™ signal proteins

Catalog Number: (786-250)
Supplier: G-Biosciences
Description: Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.
UOM: 1 * 1 KIT
Product Image-786-277

Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits

Supplier: G-Biosciences
Description: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
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Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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