Dispase, ACF
Supplier: STEMCELL TechnologiesTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Animal component-free non-specific protease.
- Obtain cleaner cultures with an animal component-free solution
- Maintain membrane integrity with this mild proteolytic enzyme
- Use this gentle reagent for isolation and passaging of primary cells
Use Dispase, Animal Component-Free (ACF) for tissue dissociation and cell isolation procedures. This neutral amino-endoprotease cleaves the N-terminal peptide bond of non-polar amino acid residues, and is obtained from cultures free of animal-derived materials. The mild proteolytic activity of Dispase makes it especially useful for the isolation and passaging of primary cells, and facilitates maintenace of membrane integrity. Dispase is commonly used in combination with other proteases such as collagenase in cell isolation and tissue dissociation for many cell types: neural tissue (Dietrich et al.), kidney (Presnell et al.), epithelial tissue (Smoot et al.), endothelial tissue (Müller et al.), lung (Barkauskas et al.), colon (Roig et al.), and stem cells (Thomson et al.; Salmon et al.).
Caution: Product Restrictions: (i) Customer shall not make products or any portion of them, in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier; and (ii) Customer is familiar with Supplier's restrictions on the use of the Products and shall use the Products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').
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