Library Preparation
sparQ DNA Library Prep kit
sparQ DNA Library Prep Kit is an optimised kit for rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on IlluminaR NGS platforms, and with input amounts from 250 pg - 1 µg.
The streamlined workflow can be completed in under 3 hours with minimal hands-on time. A single tube is used for DNA polishing, ligation, and cleanup. A second tube is used for workflows requiring PCR amplification and a final tube receives the sequencing-ready library.
Technical Literature
Product Flyer - sparQ DNA Library Prep Kit
sparQ DNA Library Prep Kit contains:
- DNA Polishing Enzyme Mix
- DNA Polishing Buffer
- DNA Ligase
- DNA Rapid Ligation Buffer
- HiFi PCR Master Mix (2X)
- Primer Mix