Description: Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods (chemiluminescence, chromogenic, and fluorescence), and the ability to immobilise proteins, glycoproteins, or nucleic acids. Protein immobilisation is thought to occur by hydrophobic interactions, and high salt and low methanol concentrations help improve protein immobilisation to the membrane during electrophoretic transfer, especially for proteins with higher molecular weights. Nitrocellulose membranes are not optimal for electrophoretic transfer of nucleic acids, as the high salt concentrations that are required for efficient binding will effectively elute some or all of the charged nucleic acid fragments.

Catalog Number: PIER88013

UOM: 1 * 15 items

Supplier: Thermo Fisher Scientific

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Description: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS)

Catalog Number: PIER28300

UOM: 1 * 5 g

Supplier: Thermo Fisher Scientific

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Description: Pierce™ HRP conjugated avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates.

Catalog Number: PIER21123

UOM: 1 * 2 mg

Supplier: Thermo Fisher Scientific

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Description: The 1-Step™ ABTS (2,2'-azinobis [3-ethylbenzthiazoline-6-sulfonic acid] diammonium salt) is a ready-to-use, water-soluble ELISA substrate for horseradish peroxidase. This substrate yields a green product with absorbance maxima at 410 nm and 650 nm. Adding SDS to the substrate stops the reaction without changing the colour.

Catalog Number: PIER37615

UOM: 1 * 250 mL

Supplier: Thermo Fisher Scientific

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Description: Pierce™ Recombinant Peroxidase Conjugated Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Catalog Number: PIER32400

UOM: 1 * 1 mg

Supplier: Thermo Fisher Scientific

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Description: Pierce silver stain for mass spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis. This kit enables both first-time and experienced users to achieve consistent and reliable staining using high, low, and gradient-percentage gels in 1D and 2D formats. The optimised staining method ensures extremely sensitive staining while minimizsing covalent crosslinking of protein to the gel matrix, which can inhibit protein recovery. The destaining reagents facilitate complete removal of silver from stained protein bands and maximum protein recovery for subsequent mass spectrometry analysis.

Catalog Number: PIER24600

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: Pierce™ Protein L chromatography cartridges are convenient, ready-to-use prepacked devices for isolation and purification of immunoglobulin classes IgG, IgM, IgA, IgE and IgD via their kappa light chains.

Catalog Number: PIER89928

UOM: 1 * 1 SET

Supplier: Thermo Fisher Scientific

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Description: Pierce™ Protein A chromatography cartridges are convenient, ready to use pre-packed devices for the isolation and purification of polyclonal and monoclonal antibodies. These cartridges are effective for routine affinity purification of polyclonal and monoclonal IgG from serum, ascites and cell culture supernatant.

Catalog Number: PIER89925

UOM: 1 * 5 mL

Supplier: Thermo Fisher Scientific

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Description: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.

Catalog Number: PIER90056

UOM: 1 * 100 µG

Supplier: Thermo Fisher Scientific

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Description: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.

Catalog Number: PIER90053

UOM: 1 * 2 µG

Supplier: Thermo Fisher Scientific

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Description: The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Catalog Number: PIER35081

UOM: 1 * 1 KIT

Supplier: Thermo Fisher Scientific

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Description: E-64 Protease Inhibitor

Catalog Number: PIER78434

UOM: 1 * 10 mg

Supplier: Thermo Fisher Scientific

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Description: Thermo Scientific Pierce SDA (NHS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 3.9Å spacer arm separates the two photoreactive groups.

Catalog Number: PIER26167

UOM: 1 * 50 mg

Supplier: Thermo Fisher Scientific

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Description: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.

Catalog Number: PIER90054

UOM: 1 * 50 µG

Supplier: Thermo Fisher Scientific

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Description: Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Catalog Number: PIER21580

UOM: 1 * 50 mg

Supplier: Thermo Fisher Scientific

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Description: Ethylene glycol

Catalog Number: PIER29810

UOM: 1 * 200 mL

Supplier: Thermo Fisher Scientific

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