You Searched For: Chiral+Technologies

Catalog Number: (STMC100-1402)

Supplier: STEMCELL Technologies

Description: Stimulating T cells with HHV6 (U90) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV6 (U90) Peptide pool is a lyophilised mixture of 267 peptides from protein U90 of human herpesvirus 6B (HHV-6B; strain Z29) and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 1078 on U90. U89 - U90 RNA expression occurs under immediate-early conditions of infection (Mirandola <i>et al.</i>), and the associated protein exhibits peri-nuclear and cytoplasmic localization (Nikolaou <i>et al.</i>). Also known as immediate-early protein IE1, latency-associated transcripts of HHV6 may function as a source of IE1 during viral reactivation (Kondo <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

UOM: 1 * 1 AU

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Supplier: STEMCELL Technologies

Description: Bone morphogenetic protein 2 (BMP-2) is a member of the transforming growth factor beta (TGF-β) superfamily. BMP-2 is a disulfide-linked homodimer, acts as a ligand for complexes of type I and II BMP receptors, and primarily activates SMAD1/5/8 signaling (Nohe <i>et al.</i>). BMP-2 is a potent differentiation factor and directs human pluripotent stem cells (hPSCs) towards various cell types including extra-embryonic endoderm, mesenchymal cells, and chondrocytes (Pera <i>et al.</i>). Although BMP-2 expression is low in healthy cartilage, its expression is upregulated at the site of cartilage damage (Blaney Davidson <i>et al.</i>). BMP-2 induces bone and cartilage formation in vitro and is able to induce chondrogenesis in human mesenchymal stem cells (Schmitt <i>et al.</i>). This product is animal component-free.

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Supplier: STEMCELL Technologies

Description: Interleukin 1 alpha (IL-1α) is a member of the IL-1 family and a dual-function cytokine. Both the unprocessed precursor and a processed IL-1α protein signal through IL-1 receptor type 1 (IL-1R1). Various cells, including keratinocytes, thymic epithelium, hepatocytes, endothelial cells, fibroblasts, and the epithelial cells of mucous membranes, have high levels of intracellular IL-1α precursor. The precursor is also expressed on the surface of monocytes and B lymphocytes (Netea <i>et al.</i>). IL-1α recruits infiltrating cells to a site of injury during necrosis and plays an important role during processes of sterile inflammation (Cohen <i>et al.</i>; Rider <i>et al.</i>). During hypoxia, IL-1α contributes to angiogenesis (Carmi <i>et al.</i>). Studies in mice show that IL-1α is produced by microglia-like cells after ischemic brain injury, which contributes to the inflammation (Luheshi <i>et al.</i>).

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Supplier: STEMCELL Technologies

Description: Interleukin 1 beta (IL-1β) is synthesized as an inactive precursor protein or pro-IL-1β. This precursor is cleaved intracellularly by caspase 1 (IL-1β convertase) to form the active form of the protein that is later secreted (Allan <i>et al.</i>). IL-1β binds to IL-1 receptor and activates intracellular signaling via the MAPK or NF-κB pathway. IL-1β is released by monocytes, tissue macrophages, and dendritic cells in response to infection or injury and induces expression of acute-phase proteins. It also promotes the infiltration of inflammatory and immunocompetent cells from the circulation into the extravascular space and affected tissues, by stimulating the expression of adhesion molecules on endothelial cells. IL-1β also affects other immune cells; for example, it co-stimulates T cell functions together with an antigen or mitogen. It also stimulates Th17 differentiation and B cell proliferation in an IL-6-dependent manner.

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Supplier: Oxford Nanopore Technologies

Description: MinION™ - your personal, portable DNA and RNA sequencer.

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Catalog Number: (STMC100-1295)

Supplier: STEMCELL Technologies

Description: Lipocalin-2 (LCN2) is a member of the lipocalin superfamily, which share a highly conserved fold containing an eight-stranded antiparallel beta barrel (Flower), and transport small hydrophobic molecules. Playing a role in innate immunity, Lipocalin-2 acts as a potent bacteriostatic reagent by sequestering iron-containing siderophores (Flo <i>et al.</i>). It is mainly expressed in activated neutrophils, but is also secreted by other immune cells in response to infection. Lipocalin-2 has been implicated in multiple cellular processes, such as differentiation (Yang <i>et al.</i>), migration (Du <i>et al.</i>), and apoptosis (Devireddy <i>et al.</i>). <i>In vitro</i> studies suggest that lipocalin-2 is a potential therapeutic target for certain cancers, including colorectal cancer (CRC; Zhang <i>et al.</i>) and chronic lymphocytic leukemia (Bauvois <i>et al.</i>). This protein product contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, lipocalin-2 from STEMCELL comes lyophilised with ≥94% purity, and endotoxin levels are verified to be ≤1.0 EU/μg protein.

UOM: 1 * 100 µG

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Catalog Number: (STMC100-1393)

Supplier: STEMCELL Technologies

Description: Stimulating T cells with HPV16 (L2) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. A lyophilised mixture of 116 peptides from minor capsid protein L2 of human papillomavirus type 16 (HPV16), HPV16 (L2) Peptide Pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 473 on L2. L2 is equipped with multiple nuclear retention signals (Darshan <i>et al.</i>; Mamoor <i>et al.</i>), and enters the nuclei of host cells via several import pathways mediated by Kapα2β1 heterodimers, Kapβ2, and Kapβ3 (Darshan <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

UOM: 1 * 1 AU

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Catalog Number: (STMC100-1398)

Supplier: STEMCELL Technologies

Description: Stimulating T cells with BKV (LT) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. BKV Peptide pool is a lyophilised mixture of 171 peptides from the Large T (LT) antigen of BK polyomavirus (BKV), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 695 on LT antigen. LT antigen is an early viral protein (Low <i>et al.</i>) that contributes to the transformation and maintenance of host cells (Nakshatri <i>et al.</i>). It has been found to bind to a subset of tumor suppressor proteins (Harris <i>et al.</i>), and its co-expression with p53 has been observed in polyomavirus-associated nephropathy (Seemayer <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

UOM: 1 * 1 AU

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Catalog Number: (STMC100-1297)

Supplier: STEMCELL Technologies

Description: A member of the pentraxin family of proteins, C-reactive protein (CRP) plays important roles in host defenses, including regulating complement activation in vivo (Sjoberg <i>et al.</i>), decreasing nitric oxide production, and inhibiting angiogenesis in vitro (Verma <i>et al.</i>, 2002). At specific concentrations, CRP directly inhibits endothelial progenitor cell (EPC) differentiation, survival, and function, and is thus implicated in the development of cardiovascular diseases (Verma <i>et al.</i>, 2004). CRP is primarily synthesized by liver hepatocytes, but also by other immune cells, such as macrophages and lymphocytes (Sproston and Ashworth). The expression of this acute-phase protein is induced by IL-6 and IL-1 secretion of macrophages and T-cells during inflammation. For consistency and reproducibility across your applications, C-reactive protein from STEMCELL comes lyophilised with ≥87% purity, and endotoxin levels are verified to be ≤1,0 EU/μg protein.

UOM: 1 * 200 µG

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Supplier: STEMCELL Technologies

Description: Vascular endothelial growth factor D (VEGF-D) is a member of the VEGF/platelet-derived growth factor (PDGF) family of proteins. VEGF-D is a potent angiogenic factor and promotes lymphangiogenesis, endothelial cell growth and survival, and can affect blood vessel permeability. VEGF-D is expressed in the lung, heart, small intestine, fetal lung, and at lower levels in the pancreas, colon, and skeletal muscle (Otrock <i>et al.</i>; Roy <i>et al.</i>; Stacker <i>et al.</i>; Yamada <i>et al.</i>). VEGF-D is a ligand for VEGF receptors 2 (Flk1) and 3 (Flt4) (Baldwin <i>et al.</i>). VEFGR-3 is highly expressed in lymphatic endothelial cells and is essential for their growth and differentiation (Otrock <i>et al.</i>; Roy <i>et al.</i>). Binding of VEGF-D to neuropilins contributes to VEGFR-3 signaling during lymphangiogenesis, whereas binding to integrin α9β1 promotes endothelial cell adhesion and migration (Roy <i>et al.</i>; Otrock <i>et al.</i>). During embryogenesis, VEGF-D also plays a role in the formation of the venous and lymphatic systems.

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Supplier: STEMCELL Technologies

Description: Heparin-binding epidermal growth factor (EGF)-like growth factor (HBEGF) is a member of the EGF family (Nishi and Klagsbrun). HBEGF promotes blastocyst adhesion to the uterine wall (Iwamoto and Mekada). It also plays a role in smooth muscle cell hyperplasia and brain injury (Nishi and Klagsburn). HBEGF produced by CD4+ T cells promotes wound healing by stimulating migration and proliferation of keratinocytes, fibroblasts, and smooth muscle cells (Blotnick <i>et al.</i>). It binds to EGFR, ErbB4, ErbB2, and ErbB3, activating the PI3K/AKT signaling cascade (Iwamoto and Mekada). HBEGF is produced in a variety of cells, where it contributes to physiological and pathological processes. HBEGF is overexpressed in ovarian, breast, gastric, colorectal, pancreatic, and endometrial cancers, which likely contributes to pathogenesis (Miyata <i>et al.</i>). This product is animal component-free.

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Catalog Number: (STMC100-1396)

Supplier: STEMCELL Technologies

Description: Stimulating T cells with the HPV18 (L1) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. A lyophilised mixture of 140 peptides from major capsid protein L1 of human papillomavirus type 18 (HPV18), HPV18 (L1) Peptide pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 568 on L1. L1 enters the nuclei of host cells and can assemble into virus-like particles (Stauffer <i>et al.</i>). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

UOM: 1 * 1 AU

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Catalog Number: (STMC01737)

Supplier: STEMCELL Technologies

Description: Perform total nucleic acid extraction from a variety of sample types using the ErythroClear™ magnet.

UOM: 1 * 1 items

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Supplier: STEMCELL Technologies

Description: For detection and measurement of mouse interferon-gamma.

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Supplier: STEMCELL Technologies

Description: Platelet-derived growth factor (PDGF) is a dimeric glycoprotein consisting of two disulfide bridge-stabilized polypeptide chains, A and B, which are assembled as heterodimers (PDGF-AB) or homodimers (PDGF-AA and PDGF-BB) (Fretto <i>et al.</i>; Westermark and Heldin). PDGF signals through the receptor tyrosine kinases PDGFRalpha and PDGFRbeta. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src, and PI3K/AKT (Kim <i>et al.</i>). PDGF is a potent mitogen for cells of mesenchymal origin, such as fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto <i>et al.</i>; Sachinidis <i>et al.</i>). It has been suggested that PDGF-AA is an important autocrine regulator of vascular endothelial growth factor (VEGF) expression in non-small cell lung carcinomas (Shikada <i>et al.</i>). PDGF-AA also mediates proliferation of oligodendrocyte progenitor cells and oligodendrocyte lineage differentiation through the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) (Hu <i>et al.</i>). PDGF-AA is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into oligodendrocyte precursor cells (Piao <i>et al.</i>).

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Supplier: STEMCELL Technologies

Description: Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf and Nicola). G-CSF causes a range of effects including a transient reduction of SDF-1 expression (Petit <i>et al.</i>), the activation of metalloproteases that cleave VCAM-1 (Levesque <i>et al.</i>), and the release of norepinephrine from the sympathetic nervous system (Katayama <i>et al.</i>), leading to the release or mobilisation of hematopoietic stem cells from the bone marrow into the periphery. The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. Binding of G-CSF to its receptor leads to activation of the JAK/STAT, MAPK, PI3K, and AKT signal transduction pathways. This product is animal component-free.

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