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Supplier: SGI - DNA Synthetic Genomics
Description: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

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Catalog Number: (SYGEGA1000-05C)
Supplier: SGI - DNA Synthetic Genomics
Description: Gibson Assembly® Positive Control, 5 reactions
UOM: 1 * 5 Tests


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Supplier: SGI - DNA Synthetic Genomics
Description: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

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Supplier: SGI - DNA Synthetic Genomics
Description: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

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Supplier: SGI - DNA Synthetic Genomics
Description: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Catalog Number: (GA2100-S)
Supplier: SGI - DNA Synthetic Genomics
Description: The Gibson Assembly® Method is a well-established assembly reaction that can be leveraged to join multiple, mutagenised DNA fragments with overlapping ends. Following mutagenesis, DNA fragments of various lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach lends itself to small and large constructs and encompasses both single and multiple insert assemblies.
UOM: 1 * 5 Tests

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Supplier: SGI - DNA Synthetic Genomics
Description: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

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Supplier: Merck
Description: Lipid removal agent is a synthetic adsorbent of crystalline calcium silicate hydrate which is used for removing lipids and endotoxin from plasma or aqueous solutions. It is also used for DNA purification to remove protein, RNA and genomic DNA.

Catalog Number: (13-3096-02)
Supplier: VWR Chemicals
Description: Fast, easy, and economical kit for isolation of genomic DNA from small sample volumes and sizes for consistent PCR and analytical test results.
UOM: 1 * 200 Tests

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Supplier: VWR Chemicals
Description: Bacterial DNA Mini Kit for the extraction of high quality DNA from gram-positive and gram-negative bacteria. For up to 1×10⁹ cells per preparation typical yield is up to 30 µg.

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Supplier: VWR Chemicals
Description: Plant RNA Kit efficiently isolates RNA from a wide range of plant species and plant tissue, effectively removing genomic DNA, polysaccharides, phenol and other secondary metabolites. Starting amount can be up to 100 mg plant tissue. The isolated RNA is directly suitable for downstream applications.

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Supplier: VWR Chemicals
Description: Fast, easy, and economical kit for isolation of DNA from a variety of samples including fresh or frozen cultured cells, tissue, blood, buccal swabs and saliva.
Supplier: VWR Chemicals
Description: Purifies DNA bands 70 bp to 20 kb in length from agarose gels. The DNA band of interest is excised from the gel, dissolved in binding buffer, and efficiently processed in a peqGOLD DNA column. >90% recovery. Elution volume: 30 to 50 µl.

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Supplier: VWR Chemicals
Description: VWR® ExoCleanUp FAST PCR reagent is a one-step PCR clean-up reagent for optimal sequencing results, consisting of a balanced combination of a heat-labile exonuclease I (HL-ExoI) and a recombinant shrimp alkaline phosphatase (rSAP).
Catalog Number: (ENZOENZ450120010)
Supplier: ENZO LIFE SCIENCES
Description: BIOARRAY™ Methylated DNA IP Kit features IP technology for the enrichment of 5-methylcytosine-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis in the completion of diverse downstream applications.
UOM: 1 * 10 Tests

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Supplier: VWR Chemicals
Description: Purification of single- or double-stranded DNA from PCR or other enzymatic reactions.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
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Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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