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AMPIGENE® 1-Step RT-PCR kit

Supplier: ENZO LIFE SCIENCES
Description: AMPIGENE® 1-Step RT-PCR Kit for optimised and efficient cDNA synthesis and PCR in a single tube.

New Product

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cDNA synthesis kits, AffinityScript

Supplier: AGILENT
Description: Provides the highest efficiency of conversion of RNA to cDNA, fully optimised for qPCR applications.

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Product Image-733-2263

qScript™ XLT One-Step RT-PCR kit

Supplier: Quantabio
Description: The qScript™ XLT One-Step RT-PCR Kit is a convenient and highly sensitive system for amplification of RNA templates up to 2 kb. cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. This system has been optimised to deliver maximum endpoint RT-PCR efficiency, sensitivity, and specificity.

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Product Image-733-2374

qScript™ XLT cDNA SuperMix

Supplier: Quantabio
Description: qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).

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Product Image-ENZOENZKIT1060050

AMPIGENE® cDNA synthesis kit

Supplier: ENZO LIFE SCIENCES
Description: AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.

New Product

Product Image-733-2905

cDNA synthesis, qScript™ Ultra SuperMix

Supplier: Quantabio
Description: Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

Product Image-733-1177

cDNA synthesis kits, qScript™

Supplier: Quantabio
Description: qScript™ cDNA synthesis kit contains qScript™ Reverse Transcriptase, a mixture of a MMLV reverse transcriptase derivative and ribonuclease inhibitor protein, optimised for sensitive and reliable cDNA synthesis over a broad dynamic range of input RNA. cDNA synthesis reagents are provided in a variety of SuperMix and kit formats to suit specific applications.

SDS

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miRNA QRT-PCR detection reagent

Catalog Number: (AGLS600584)
Supplier: AGILENT
Description: Polyadenylation of miRNA from total RNA and synthesis of first-strand cDNA from tailed miRNAs.
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AMPIGENE® qPCR 1-Step Probe Kit No-ROX

Supplier: ENZO LIFE SCIENCES
Description: Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

New Product

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AMPIGENE® qPCR 1-Step Probe Kit Lo-ROX

Supplier: ENZO LIFE SCIENCES
Description: Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

New Product

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AMPIGENE® qPCR 1-Step Green Kit Hi-ROX

Supplier: ENZO LIFE SCIENCES
Description: Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

New Product

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AMPIGENE® qPCR 1-Step Green Kit Lo-ROX

Supplier: ENZO LIFE SCIENCES
Description: Optimised and efficient cDNA synthesis and dye-based qPCR in a single tube.

New Product

Product Image-733-2469

Ultra-Plex™ 1-Step ToughMix® (4X)

Supplier: Quantabio
Description: Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

SDS Certificates

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AMPIGENE® qPCR 1-Step Probe Kit Hi-ROX

Supplier: ENZO LIFE SCIENCES
Description: Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

New Product

Product Image-733-1259

qScript™ One-Step qRT-PCR kits

Supplier: Quantabio
Description: Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ <i>Taq</i> DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified <i>Taq</i> DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.

Certificates

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Maxima™ H minus cDNA synthesis master mix, with dsDNase

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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