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Product Image-PIER89839

Organelle Enrichment Kits

Catalog Number: (PIER89839)
Supplier: Thermo Fisher Scientific
Description: Organelle enrichment kit for lysosomes enables enrichment of intact organelles from cells and tissue. The kit provides the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation.
UOM: 1 * 1 KIT
Product Image-786-316

Cell surface protein isolation and labelling kit, HOOK™

Catalog Number: (786-316)
Supplier: G-Biosciences
Description: The HOOK™ Cell Surface Protein Isolation Kit uses HOOK™ biotin labelling and purification technology in conjunction with Mammalian Cell PE LB™ Lysis Buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. The Kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines.
UOM: 1 * 1 KIT
Image Unavailable-116544050.

SPINeasy DNA/RNA/protein all in one kit, 50 preparations, MP Biomedicals

Catalog Number: (116544050.)
Supplier: MP Biomedicals
Description: Quick and efficient isolation of DNA, RNA and protein from the same sample using spin column technology.
UOM: 1 * 50 Tests
Product Image-PIER88305

Detergent removal spin column kit, HiPPR™

Supplier: Thermo Fisher Scientific
Description: The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.

Product Image-M04018BF

VWR®, DNA extraction Kit for Real-time PCR food samples analysis

Catalog Number: (M04018BF)
Supplier: VWR Chemicals
Description: Rapid method for DNA extraction and purification from all types of microorganisms in a variety of matrices for molecular biology.
UOM: 1 * 1 KIT

Certificates


Product Image-PIER89805

Glycoprotein Isolation Kit, Cona And WGA, Pierce™

Supplier: Thermo Fisher Scientific
Description: The glycoprotein isolation kit allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) or wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples - those containing alpha-lined mannose or terminal glucose residues (in case of ConA) and N-Acetyl glucosamine (GlcNAc) groups or sialic acid (in case of WGA). This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Image Unavailable-ENZOBMLKI1040050

Fluor-de-Lys® Deacetylase Substrate

Catalog Number: (ENZOBMLKI1040050)
Supplier: ENZO LIFE SCIENCES
Description: Fluor de Lys® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the Fluor de Lys® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the Fluor de Lys® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with Fluor de Lys® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).
UOM: 1 * 50 µl
Product Image-PIER84850

Accessories for mass spectrometry sample preparation kit

Catalog Number: (PIER84850)
Supplier: Thermo Fisher Scientific
Description: Pierce™ C18 spin tips, for elution of up to 10 µg peptides per 20 µl sample for MS
UOM: 1 * 1 KIT
Product Image-PIER78835

Nuclear and cytoplasmic extraction reagents, NE-PER

Supplier: Thermo Fisher Scientific
Description: The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Product Image-13-3486-00

Peqlab peqGOLD, DNA Isolation, Plant DNA Mini Kit

Supplier: VWR Chemicals
Description: Dependable isolation of high quality total cellular DNA from a variety of plant species and tissues. peqGOLD homogeniser columns remove cell debris and improve sample handling following lysis.

SDS Certificates

Product Image-13-6943-00

VWR® Peqlab peqGOLD Plasmid Purification, Plasmid Miniprep Kit I

Catalog Number: (13-6943-00)
Supplier: VWR Chemicals
Description: Plasmid Miniprep Kit I for the easy, quick and efficient extraction of highly pure plasmid DNA. Isolate up to 30 µg of high quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA obtained with peqGOLD Plasmid Miniprep kits can directly be used for a wide range of downstream applications.
UOM: 1 * 5 Tests

SDS Certificates


Product Image-13-1091-00

Peqlab peqGOLD, DNase I Digest Kit

Catalog Number: (13-1091-00)
Supplier: VWR Chemicals
Description: The peqGOLD DNase I Digest Kit is optimised for use with peqGOLD RNA extraction protocols. Normally DNase I digestion is not required for RNA purified with peqGOLD RNA Mini Columns as our silica-based spin column technology efficiently removes the majority of DNA without enzymatic digestion. However, certain sensitive RNA applications may require further DNA removal.
UOM: 1 * 5 Tests

SDS Certificates


Product Image-13-B2013-02

VWR®, Accessories for peqGOLD Kits

Supplier: VWR Chemicals
Description: Nucleic Acid Purification Kits and Reagents, Solution I

SDS Certificates

Product Image-13-2500-01

Peqlab peqGOLD, DNA Clean-up, Gel Extraction Kits

Catalog Number: (13-2500-01)
Supplier: VWR Chemicals
Description: Purifies DNA bands 70 bp to 20 kb in length from agarose gels. The DNA band of interest is excised from the gel, dissolved in binding buffer, and efficiently processed in a peqGOLD DNA column. >90% recovery. Elution volume: 30 to 50 µl.
UOM: 1 * 50 Tests

SDS Certificates


Product Image-13-6492-02

Peqlab peqGOLD Cycle-Pure™, PCR Clean-up, Kits

Supplier: VWR Chemicals
Description: Purification of single- or double-stranded DNA from PCR or other enzymatic reactions.

SDS Certificates

Product Image-733-2879

VWR® Fast extract genotyping PCR kit

Supplier: VWR Chemicals
Description: This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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