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Product Image-78840.

Subcellular Protein Fractionation Kit for Cultured Cells

Catalog Number: (78840.)
Supplier: Thermo Fisher Scientific
Description: This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
UOM: 1 * 1 KIT
Product Image-786-177

Protein extraction kit, bacterial PE LB™

Supplier: G-Biosciences
Description: Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Image Unavailable-69022-3

Thrombin cleavage capture kit, Millipore®

Catalog Number: (69022-3)
Supplier: Merck Millipore (Novagen)
Description: The thrombin cleavage capture kit is designed for cleavage of fusion proteins followed by convenient and quantitative removal of thrombin protease. The method is based on the use of biotinylated thrombin for digestion and its subsequent removal with streptavidin agarose.
UOM: 1 * 1 KIT
Product Image-786-241A

InGel™ Array kit for protein digestion

Catalog Number: (786-241A)
Supplier: G-Biosciences
Description: InGel™ Array is a 96-well format kit suitable for processing larger numbers of protein spots concurrently and is compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste.
UOM: 1 * 1 KIT
Product Image-786-179

Protein extraction kit, yeast PE LB™

Supplier: G-Biosciences
Description: Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.

Product Image-786-126

Protein concentration kit, Column-PROTEIN-Concentrate™

Catalog Number: (786-126)
Supplier: G-Biosciences
Description: The Column-PROTEIN-Concentrate™ Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilises any protein in a low salt buffer between pH 2 to 12. The binding capacity is ~0,5 mg protein/ml Protein Binding Resin.
UOM: 1 * 1 KIT

SDS


Product Image-PIER87791

Subcellular protein fractionation kit for tissues

Supplier: Thermo Fisher Scientific
Description: This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

Product Image-PIER89875

Albumin/IgG removal kit, Pierce™

Catalog Number: (PIER89875)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.
UOM: 1 * 1 KIT
Product Image-786-120

Protein precipitation and concentration kit, UPPA-PROTEIN-Concentrate™

Supplier: G-Biosciences
Description: UPPA PROTEIN-Concentrate™ Kit uses a proprietary reagent UPPA™ (Universal Protein Precipitation Agent) to quantitatively concentrate dilute protein samples as low as 1 ng/ml. Concentration is not affected by the presence of common laboratory agents, including detergents and chaotropes. After precipitation, the sample is washed to remove salts and other interfering agents; complete recovery of sample is produced.

Product Image-PIER89801

Mitochondria Isolation Kit for Tissue

Catalog Number: (PIER89801)
Supplier: Thermo Fisher Scientific
Description: The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour.
UOM: 1 * 1 KIT
Product Image-786-316

Cell surface protein isolation and labelling kit, HOOK™

Catalog Number: (786-316)
Supplier: G-Biosciences
Description: The HOOK™ Cell Surface Protein Isolation Kit uses HOOK™ biotin labelling and purification technology in conjunction with Mammalian Cell PE LB™ Lysis Buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. The Kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines.
UOM: 1 * 1 KIT
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Phosphoserine detection set

Catalog Number: (ENZOALX850023KI01)
Supplier: ENZO LIFE SCIENCES
Description: The kit offers a set of six different MAbs specific for serine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries. Clone 16B4 strongly interacts with pSK and pSP motifs, thus specifically recognizes substrates of MAP/SAP Kinases and CDC kinases.Clones 1C8, 4A3, 4A9, 4H4 and 7F12 interact with epitopes containing phosphoserine in the context of positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.) Though revealing a similar pattern in epitope mapping using phosphopeptide libraries, these antibodies interact with different sets of phosphoproteins in whole cell lysates.
UOM: 1 * 1 SET
Product Image-PIER85160

Albumin depletion kit, Pierce™

Catalog Number: (PIER85160)
Supplier: Thermo Fisher Scientific
Description: Pierce™ Albumin Depletion Kit improves serum component analysis by rapidly removing abundant albumin protein from serum samples. The albumin depletion resin provided in the kit is a high capacity, immobilised Cibacron Blue dye agarose resin, which binds a variety of species-specific albumins.
UOM: 1 * 1 KIT
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Fluor-de-Lys® Deacetylase Substrate

Catalog Number: (ENZOBMLKI1040050)
Supplier: ENZO LIFE SCIENCES
Description: Fluor de Lys® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the Fluor de Lys® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the Fluor de Lys® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with Fluor de Lys® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).
UOM: 1 * 50 µl
Product Image-786-219

Extraction buffers, FOCUS™

Supplier: G-Biosciences
Description: FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

Product Image-PIER89874

Mitochondria isolation kit for cultured cells

Catalog Number: (PIER89874)
Supplier: Thermo Fisher Scientific
Description: The Mitochondria isolation Kit for cultured cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
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Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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