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Fluor-de-Lys® Developer I concentrate

Catalog Number: (ENZOBMLKI1050300)
Supplier: ENZO LIFE SCIENCES
Description: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
UOM: 1 * 300 µl
Product Image-786-077

Protein reducing agents

Supplier: G-Biosciences
Description: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Product Image-PIER88279

Mouse embryonic fibroblast isolation kit, Pierce™

Catalog Number: (PIER88279)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.
UOM: 1 * 1 KIT
Product Image-786-273

Antibody fab fragmentation kit

Supplier: G-Biosciences
Description: Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.

Image Unavailable-ENZOBMLPW1075A0001

Proteasome Purification Kit

Catalog Number: (ENZOBMLPW1075A0001)
Supplier: ENZO LIFE SCIENCES
Description: Proteasome Purification Kit is a high integrity kit for the isolation and purification of proteasome complexes from biological samples such as cell lysates, tissue extracts, plasma, serum, confirmed by Western blot analysis.
UOM: 1 * 10 Tests
Product Image-786-088

Carrier proteins

Supplier: G-Biosciences
Description: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Product Image-BB01

Botanical garden blot

Catalog Number: (BB01)
Supplier: G-Biosciences
Description: G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.
UOM: 1 * 1 items
Product Image-92076.

CF® Dye BCN

Supplier: Biotium
Description: CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labelling reactions are also available.

Image Unavailable-ENZOBMLKI1761250

Fluor-de-Lys® Developer II concentrate

Catalog Number: (ENZOBMLKI1761250)
Supplier: ENZO LIFE SCIENCES
Description: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
UOM: 1 * 1,25 mL
Image Unavailable-ENZOALX850024KI01

Phosphothreonine detection set

Catalog Number: (ENZOALX850024KI01)
Supplier: ENZO LIFE SCIENCES
Description: This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site.
UOM: 1 * 1 SET
Image Unavailable-BOSSBS-8376R-A750

Anti-RNF160 Rabbit Polyclonal Antibody (Alexa Fluor® 750)

Catalog Number: (BOSSBS-8376R-A750)
Supplier: Bioss
Description: E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesised nascent chains for proteasomal degradation (PubMed:23685075, PubMed:25132172, PubMed:25578875). Ubiquitination leads to VCP/p97 recruitment for extraction and degradation of the incomplete translation product (By similarity).
UOM: 1 * 100 µl
Image Unavailable-BOSSBS-8376R-A680

Anti-RNF160 Rabbit Polyclonal Antibody (Alexa Fluor® 680)

Catalog Number: (BOSSBS-8376R-A680)
Supplier: Bioss
Description: E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesised nascent chains for proteasomal degradation (PubMed:23685075, PubMed:25132172, PubMed:25578875). Ubiquitination leads to VCP/p97 recruitment for extraction and degradation of the incomplete translation product (By similarity).
UOM: 1 * 100 µl
Image Unavailable-ENZOADI900199

[pSer326]HSF1 ELISA Kits

Catalog Number: (ENZOADI900199)
Supplier: ENZO LIFE SCIENCES
Description: The HSF1 EIA kit is a complete kit for the quantitative determination of HSF1 phosphorylated at Ser326 in cell lysates and tissue extracts from human and mouse origins. This is a sensitive ELISA kit for the key transcription factor for heat shock protein (chaperone) research.
UOM: 1 * 1 KIT
Image Unavailable-ENZOBMLUW99150001

Ubiquitin conjugating kit (HeLa lysate-based)

Catalog Number: (ENZOBMLUW99150001)
Supplier: ENZO LIFE SCIENCES
Description: 1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
UOM: 1 * 20 Tests
Image Unavailable-ENZOADI900198

HSF1 ELISA Kits

Catalog Number: (ENZOADI900198)
Supplier: ENZO LIFE SCIENCES
Description: The HSF1 EIA kit is a complete kit for the quantitative determination of HSF1 in cell lysates and tissue extracts from human and mouse origins. This is a Sensitive ELISA kit for this key transcription factor for heat shock protein (chaperone) research.
UOM: 1 * 1 KIT
Image Unavailable-APOSBIB2010-10G

n-Dodecyl-β-D-maltoside ≥99%, Ultrapure

Supplier: Apollo Scientific
Description: Non ionic detergent used to extract proteins

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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