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Neurotrophin antibody kit

Supplier: Biosensis
Description: The Neurotrophin Antibody Kit contains a collection of sheep antibodies raised against BDNF, NGF, NT3 and NT4/5. The kit is completed with one vial of normal sheep IgG for use as negative control. This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.

Image Unavailable-ENZOBMLUW99150001

Ubiquitin conjugating kit (HeLa lysate-based)

Catalog Number: (ENZOBMLUW99150001)
Supplier: ENZO LIFE SCIENCES
Description: 1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
UOM: 1 * 20 Tests
Image Unavailable-ENZOENZ51034K500

Green detection kit, LYSO-ID®

Supplier: ENZO LIFE SCIENCES
Description: LYSO-ID® Green Detection Kit is an acidic organelle-selective dye specially designed to use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).

Image Unavailable-ENZOBMLP2660001

CDK2 substrate,Biotin

Catalog Number: (ENZOBMLP2660001)
Supplier: ENZO LIFE SCIENCES
Description: This peptide, Biotin-Axh-HHASPRK, is a specific substrate for CDK2. The biotin allows the peptide to be used in kinase assays with streptavidin-bound membranes.
UOM: 1 * 1 mg
Product Image-MLDVR8088

Cyclic-AMP fluorescent assay kits, CatchPoint™

Supplier: Molecular Devices
Description: CatchPoint™ cAMP Fluorescent Assay Kit measures cAMP levels via a competitive immunoassay format. Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or Cyclic AMP (cAMP). Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of GPCRs.

Product Image-MLDVR8123

Membrane potential assay kit, FLIPR®

Supplier: Molecular Devices
Description: The FLIPR® Membrane Potential Assay Kit provide a fast, simple and reliable, fluorescence-based assay for detecting changes in voltage across the cell membrane and its homogenous fluorescence-based formulations for observation of real-time membrane potential changes associated with ion channel activation and ion transporter proteins.

Product Image-ENZOBMLUW09700001

Auto-Ubiquitinylation Kit

Catalog Number: (ENZOBMLUW09700001)
Supplier: ENZO LIFE SCIENCES
Description: This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.
UOM: 1 * 10 Tests
Image Unavailable-ENZOALX850229KI01

Caspase fluorometric substrate set

Catalog Number: (ENZOALX850229KI01)
Supplier: ENZO LIFE SCIENCES
Description: Fluorometric substrates for assaying activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
UOM: 1 * 1 SET
Image Unavailable-ENZOBMLAW97850005

Ac-Arg-Leu-Arg-AMC

Catalog Number: (ENZOBMLAW97850005)
Supplier: ENZO LIFE SCIENCES
Description: This fluorogenic peptide is often the substrate of choice for assaying the trypsin-like activity of purified proteasomes. Benefits include a low Km, high specific activity, and the fact that the substrate is cleaved exclusively at the R-AMC bond.
UOM: 1 * 5 mg
Product Image-29045-500UL

Annexin V conjugates

Supplier: Biotium
Description: Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

Image Unavailable-ENZOADI801928

Bovine serum albumin (BSA)

Catalog Number: (ENZOADI801928)
Supplier: ENZO LIFE SCIENCES
Description: This BSA Solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol. The BSA Solution may be used for any other assay development needs. Additional assay development reagents are available as companion products to our ImmunoSet products.
UOM: 1 * 50 mL
Product Image-MLDVR8200

Western blot assay kits, ScanLater™

Supplier: Molecular Devices
Description: The ScanLater™ Western Blot Assay Kits are a time-resolved fluorescence (TRF)-based assay, optimal for quantitating as little as femtogram protein samples.

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Red detection kit (GFP-CERTIFIED®), MITO-ID®

Supplier: ENZO LIFE SCIENCES
Description: MITO-ID® Red Detection Kit (GFP-CERTIFIED®) is a photostable, non-toxic and selective mitochondrial dye that stains live, detergent permeabilised and aldehyde fixed cells. This kit is specifically designed for use with GFP expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFP's, CFP's, YFP's).

Product Image-ROCKPA00-02

Avidin, biotin, protein A/G and streptavidin reagents

Supplier: Rockland Immunochemicals
Description: Avidin is biotin-binding protein, tetramer and suitable for used as a antigen, control and standard in assays. Avidin fluorescein, rhodamine, alkaline phosphatase, Texas Red™ conjugated are suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Avidin conjugated with glucose oxidase and peroxidase are useful as a detection reagent for primary antibodies conjugated with biotin and also can be utilised in Western blotting and ELISA experiments with proper substrates.

Image Unavailable-40100.

Dye conjugates dUTP CF®

Supplier: Biotium
Description: CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for <i>in situ</i> hybridisation and nucleic acid blotting applications.

Image Unavailable-ENZOBMLUW0400A0001

Ubiquitin activating kit

Catalog Number: (ENZOBMLUW0400A0001)
Supplier: ENZO LIFE SCIENCES
Description: Ubiquitin Activating Kit for activation of ubiquitin for use in ubiquitinylation experiments. The kit provides the means of generating thioester linked, activated ubiquitin-E1 conjugates, utilising the first step in the ubiquitin cascade, for investigation of ubiquitin activation, subsequent ubiquitin transfer to/interaction with E2 conjugating enzymes, and their use in the ubiquitinylation of E3 ligases and target substrate proteins. Ubiquitin antibody provided allows high sensitivity detection of ubiquitin and ubiquitin-conjugates.
UOM: 1 * 20 Tests
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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