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Calcium chloride ≥99%, white powder cell culture reagent

Supplier: MP Biomedicals
Description: Calcium Chloride is a salt of calcium and chloride. It behaves as a typical ionic halide, and is solid at room temperature.
Calcium chloride is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. It has been used in the stabilization and two dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from Escherichia coli. It is also used as a dessicant, coagulant in rubber manufacturing and as an accelerator in the concrete industry.

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Protamine sulphate (from salmon sperm), ≥100 USP u/mg, white powder cell culture reagent

Supplier: MP Biomedicals
Description: Storage: Room Temperature, desiccate
Protamine sulfate is a purified mixture of simple protein principles obtained from the sperm or testes of suitable species of fish, which has the property of neutralizing heparin. Because of having many basic amino acids (mostly arginine) protamine contains far greater nitrogen than other proteins. Its molecular weight is relatively small. Histone and other basic proteins in the testes of unmatured fishes convert into protamine along with the growth of the fishes. In the testes, protamine takes the form of nucleoprotamine linked with DNA.
Protamine Sulfate is a raw material for study preparations like insulin compounds, and etc. It is used in separation and refining of vaccines. It is a reagent for removing nucleic acids from enzyme solution for the purpose of easy separation and refining. Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine sulfate is a small cationic protein that binds and precipitates DNA. Inhibits lipoprotein lipase. Protamine sulfate shown to inhibit the classical pathway of complement. It inhibits turnover of lipoproteins by lipoprotein lipase.

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Sodium pyruvate ≥99%, white powder cell culture reagent

Supplier: MP Biomedicals
Description: Storage: +4 °C
Pyruvic acid is an intermediate in sugar metabolism and in enzymatic carbohydrate degradation (alcoholic fermentation) where it is converted to acetaldehyde and CO2 by carboxylase. In muscle, pyruvic acid (derived from glycogen) is reduced to lactic acid during exertion, which is reoxidized and partially retransformed to glycogen during rest. It improves coliform recovery when present in culture medium. It is involved in a metabolic regulatory pathway activated by mitochondrial oxidants. Pyruvate is involved in respiratory regulation in plants by interacting with alternative oxidase at a conserved cysteine residue. It may help prevent hydrogen peroxide mediated cell death.
Sodium pyruvate is utilized as a component in culture broth and media. Sodium pyruvate is used in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus. Sodium pyruvate has also been used to establish stably transfected human B cell lines.
Sodium Pyruvate has shown antioxidant properties and protective effects against oxygen radicals. Pyruvate is produced as part of glycolysis and is an intermediate in many metabolic pathways. It can be converted into acetyl CoA and enter the TCA Cycle.

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Mag-Bind® Endo-free Plasmid Mini Kit

Supplier: OMEGA BIO-TEK
Description: <p>The Mag-Bind® Endo-free Plasmid Mini Kit is designed to deliver high-quality, plasmid DNA with endotoxin levels <0,1 EU/μg for use in eukaryotic transfections and other sensitive <em>in vitro</em> applications.</p>

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E.Z.N.A.® Endo-Free plasmid kits

Supplier: OMEGA BIO-TEK
Description: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.

SDS

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Adenovirus purification kits, Vivapure® AdenoPACK™

Supplier: Sartorius
Description: The Vivapure® AdenoPACK™ adenovirus purification and concentration kits offer researchers who need to recover up to 3×10¹³ purified recombinant adenovirus particles for<i> in vitro</i> transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures.

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Mag-Bind® Endo-Free plasmid 96 kit

Supplier: OMEGA BIO-TEK
Description: The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.

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Human ERBB2

Catalog Number: (ORIGTS422909P5)
Supplier: OriGene
Description: HEK293T cells were transiently transfected with TrueORF cDNA plasmid. Transfected cells were cultured for 48 hrs. After harvesting, the cultured cells were fixed in formalin and dehydrated before embedding in paraffin. 5 µm sections of the FFPE cell pellet blocks are cut and mounted on positively charged SuperFrost slides.
UOM: 1 * 1 KIT
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Human ERBB2

Catalog Number: (ORIGTS412583P5)
Supplier: OriGene
Description: HEK293T cells were transiently transfected with TrueORF cDNA plasmid. Transfected cells were cultured for 48 hrs. After harvesting, the cultured cells were fixed in formalin and dehydrated before embedding in paraffin. 5 µm sections of the FFPE cell pellet blocks are cut and mounted on positively charged SuperFrost slides.
UOM: 1 * 1 KIT
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Firefly luciferase assay kit (Lyophilised)

Supplier: Biotium
Description: This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.

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Calcium chloride solution 0.5 M for molecular biology

Catalog Number: (AVIVOORA00231)
Supplier: Aviva Systems Biology
Description: 0.5 M Calcium Chloride is suitable in molecular biology assays such as genetic transfection.
UOM: 1 * 500 mL
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AmMag™ Quatro Automation Module

Catalog Number: (GENSD00019)
Supplier: Genscript
Description: The AmMag Quatro is an automated solution for purification of large-scale, high quality, transfection-grade plasmids from <i>Escherichia coli </i>cultures.
UOM: 1 * 1 items

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E.Z.N.A.® Plasmid Maxi Kit

Supplier: OMEGA BIO-TEK
Description: The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, <i>E.coli</i> strain, and conditions of growth, 50 to 200 ml of overnight culture in LB medium typically produces 0,5 to 1 mg of high copy number plasmid DNA. Up to 500 ml of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription<i> invitro</i>, and other applications.

SDS Certificates

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AmMag™ Quatro System Controllers

Catalog Number: (GENSD00018)
Supplier: Genscript
Description: The AmMag Quatro is an automated solution for purification of large-scale, high quality, transfection-grade plasmids from <i>Escherichia coli</i> cultures.
UOM: 1 * 1 items

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Aluminon, powder, Sigma-Aldrich®

Catalog Number: (A0885-25G)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Aurintricarboxylic acid ammonium salt has been used as a nuclease inhibitor to study its effects on transfection.
UOM: 1 * 25 g
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Anti-KT3 tag Rabbit Polyclonal Antibody

Catalog Number: (BOSSBS-2112R)
Supplier: Bioss
Description: KT3 epitope tag can be used to monitor expression of the protein products transfected with cDNA constructs.
UOM: 1 * 100 µl
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
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