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Chromogenic endotoxin quant kit, Pierce™

Supplier: Thermo Fisher Scientific
Description: The Thermo Scientific Pierce™ Chromogenic endotoxin Quant kit is a highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acid, or antibody sample using the amebocyte lysate assay. The kit enables detection within two linear sensitivity ranges of 0,01 to 0,1 EU/ml and 0,1 to 1,0 EU/ml.

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dNTP mix

Catalog Number: (733-1271)
Supplier: Quantabio
Description: The 10 mM dNTP mix is a solution of high purity deoxyribonucleoside triphosphates that has been functionally qualified for real-time quantitative PCR (qPCR). It is suitable for use in conventional end-point PCR, real time qPCR, first-strand cDNA synthesis, as well as other applications that require dNTP as substrate. The use of qPCR certified dNTPs is a simple but important measure to ensure consistent assay performance.
UOM: 1 * 1.000 µl

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DNA gel extraction kit

Supplier: Biotium
Description: The DNA Gel Extraction Kit is a silica-gel, spin column based DNA extraction kit designed to purify 40 bp to 40 kb DNA fragments from agarose gels in TAE or TBE buffer.

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RNA purification kit, Tri-Xtract™

Supplier: G-Biosciences
Description: Tri-Xtract™ is designed for the isolation of total RNA that is free from protein and DNA contamination. The isolated RNA is suitable for Northern blots, dot blot hybridisation, <i>in vitro</i> translation, RNase protection assays and poly (A+) selection.

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DNA transfection reagent, EcoTransfect™

Supplier: OZ BIOSCIENCES
Description: EcoTransfect™ transfection reagent is an economical lipofection reagent dedicated to the transfection of popular cell lines. It is the perfect solution for quickly analysing the biological activity of nucleic acids, to perform routine transfection assays at low cost and to accomplish high throughput screening.

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Spectrophotometers, GeneQuant™ 1300

Catalog Number: (SCLI80-2120-02)
Supplier: BIOCHROM
Description: The GeneQuant 1300 spectrophotometer is pre-programmed with methods for nucleic acid purification, bacterial cell cultures, protein assays, Cy™Dye and cell culture density measurement.
UOM: 1 * 1 items
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ABsolute™ qPCR Low ROX Mixes

Supplier: Thermo Fisher Scientific
Description: ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.

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Ethidium bromide

Catalog Number: (ICNA04802511)
Supplier: MP Biomedicals
Description: Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. A 10mg/mL easy-to-use solution of ethidium bromide in specially filtered, deionized water which is excellent for nucleic acid electrophoresis and purification applications, eliminates the dust hazard associated with powdered ethidium bromide and saves time spent on weighing and mixing. Used in the fluorometric detection of double stranded nucleic acids in gel electrophoresis. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. It confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequent fluorescence techniques enable the extraction and characterization of DNA bands. Biochem/physiol Actions: Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
UOM: 1 * 10 mL
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Anti-IFNA2 Mouse Monoclonal Antibody [clone: N27]

Catalog Number: (PRSI33-720)
Supplier: ProSci Inc.
Description: Recognises a protein of 16-27 kDa, identified as human Interferon alpha 2 (IFNA2). Its epitope maps between amino acids 43-53 of the IFNA2. This mAb is specific for IFNA2 and does not cross-react with IFNA1. The epitopes of clones N27 and N39 mAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFNA2 after viral infections. The IFNA family consists of 24 or more genes or pseudo-genes. The alpha interferons are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.
UOM: 1 * 100 µG

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One step RT-PCR master mix kit

Catalog Number: (71978-3)
Supplier: Merck Millipore (Novagen)
Description: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.
UOM: 1 * 1 KIT
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Anti-IFNA2 Mouse Monoclonal Antibody [clone: N39]

Catalog Number: (PRSI33-405)
Supplier: ProSci Inc.
Description: Recognises a protein of 16-27 kDa, identified as human interferon-II) (IFN-(II). Its epitope maps between aa112-148 of IFN-II) (total aa172). This mAb is specific for IFN-(II) and does not cross-react with IFN-(I). The site recognized by this mAb is called "site I" and is responsible for the antiviral and anti-proliferative activities of IFN-(II). Epitopes of N27 and N39 mAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-(II) after viral infections. The IFN- family consists of 24 or more genes or pseudo-genes. IFN-II) is one of the two distinct families (I and II) of human IFN-. The -interferon are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.
UOM: 1 * 100 µG

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VWR®, Accessories for peqGOLD Kits

Supplier: VWR Chemicals
Description: Nucleic Acid Purification Kits and Reagents, Solution I

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Peqlab peqGOLD Cycle-Pure™, PCR Clean-up, Kits

Supplier: VWR Chemicals
Description: Purification of single- or double-stranded DNA from PCR or other enzymatic reactions.

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Peqlab peqGOLD, DNA Isolation, Microspin Tissue DNA Mini Kits

Catalog Number: (13-3096-02)
Supplier: VWR Chemicals
Description: Fast, easy, and economical kit for isolation of genomic DNA from small sample volumes and sizes for consistent PCR and analytical test results.
UOM: 1 * 200 Tests

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VWR®, Phenol-free total RNA purification kit

Catalog Number: (N788-KIT)
Supplier: VWR Chemicals
Description: The phenol-free total RNA purification kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses.
UOM: 1 * 1 KIT

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Peqlab peqGOLD, Plasmid Purification, Plasmid Miniprep Kit II

Catalog Number: (13-6945-00)
Supplier: VWR Chemicals
Description: Isolate up to 40 to 70 µg of high quality plasmid DNA from up to 15 ml bacterial cultures in 30 minutes or less. Simplified with VWR® peqGold Mini Column II technology into three quick steps: Bind, wash and elute.
UOM: 1 * 5 Tests

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
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The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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