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Catalog Number: (208291-250)
Supplier: Merck Millipore (Calbiochem‎)
Description: Calcium chloride dihydrate ≥99% (by complexometric analysis), white solid, Molecular Biology Grade, Millipore®
UOM: 1 * 250 g


Supplier: Thermo Fisher Scientific
Description: Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) 99.8-100.1% (dry basis), Ultrapure for molecular biology
Supplier: MP Biomedicals
Description: Potassium chloride is a salt commonly used in the preparation of phosphate buffered saline and for the extraction and solubilization of proteins.It is a good potassium source. Also used in the hypotonic KCl-propidium iodide protocol for univariate analysis of metaphase chromosomes; dissociation of eukaryotic ribosomes into subunits by high concentrations of potassium (0.3-1.0 M); preparation of mitochondria, provides an ionic osmotic support for those tissues that assume a gelatinous consistency upon homogenization.

Supplier: MP Biomedicals
Description: Storage: Room Temperature
Guanidine hydrochloride consists of a network of guanidinium cations and chloride anions linked by N–H•••Cl hydrogen bonds, it is a strong chaotropic agent. Guanidine HCl may agglomerate upon storage. It may appear as a free-flowing crystalline powder, a free flowing powder with solid material dispersed throughout, or a solid. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine HCl appear identical.
Guanidine hydrochloride can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA. Guanidine hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of protein, it can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins.

Supplier: Thermo Fisher Scientific
Description: , powder

SDS

Supplier: Merck
Description: A non-ionic synthetic polymer of sucrose.

Supplier: Cytiva
Description: For determination of molecular weight of uncharacterised protein samples with precision by comparison with carefully selected standards.
Supplier: MP Biomedicals
Description: Storage: Store at Room Temperature (15-30 °C), dessicate.
Ammonium persulfate is a strong oxidizing agent. It is a radical initiator.
Ammonium persulfate is a polymerization catalyst used with TEMED for polyacrylamide gel formation. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry. Other applications of APS include its use as a reducer and retarder in photography, the manufacture of aniline dyes, electroplating, the decolorizing and deodorizing of oils.

Supplier: Merck Millipore (Calbiochem‎)
Description: HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) free acid ≥99% (by alkalimetry), Molecular Biology Grade, Millipore®
Catalog Number: (ACRO327345000)
Supplier: Thermo Fisher Scientific
Description: CAS No.: 38641-82-6
UOM: 1 * 500 mL

Catalog Number: (27-2010)
Supplier: VWR Chemicals
Description: Protein Marker II for accurate molecular weight determination of proteins by SDS-PAGE.
UOM: 1 * 1 KIT

Catalog Number: (720-2924)
Supplier: VWR Collection
Description: Set of prepared slides for educational purposes, to better understand general biology.
UOM: 1 * 1 KIT


Catalog Number: (69278-3)
Supplier: Merck Millipore (Novagen)
Description: PCR Molecular Size Marker contains a mixture of eight defined DNA fragments ranging from 50 to 2000 bp at convenient size intervals for characterising small DNA products. The markers are supplied in gel loading buffer containing two tracking dyes that do not interfere with UV illumination of ethidium bromide-stained bands. A separate vial of 6X Loading Buffer is included. Each vial of markers is sufficient for 50 lanes on TBE or TAE agarose stained with ethidium bromide. The recommended amount for loading (5 µl) produces bands of even intensity that are bright, sharp and easy to photograph.
UOM: 1 * 1 items


Catalog Number: (J67051.XI)
Supplier: Thermo Fisher Scientific
Description: This wide range molecular weight marker contains 60 ng/μl of 21 fragments from 100 bp to 12000 bp. The bands appear in 100 bp increments from 100 to 1000 bp and in 1000 bp increments from 1 to 12 kb. Bands at 1 kb and 5 kb stain brighter to aid identification.
UOM: 1 * 0,5 mL


Supplier: VWR Chemicals
Description: Our 3A molecular sieve is commonly used for drying of gases and polar liquids (methanol, ethanol) and easily polymerisable substances, such as unsaturated hydrocarbons (ethylene, propylene, acetylene and butadiene).
Supplier: MP Biomedicals
Description: Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
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The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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