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Product Image-ICNA117022600

DNA extraction kits, MPure™ Bacterial DNA extraction kit, MP Biomedicals

Catalog Number: (ICNA117022600)
Supplier: MP Biomedicals
Description: MPure™ Bacterial DNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction and purification of genomic DNA from both gram-positive and gram-negative bacteria. Nucleic acids extracted and purified from MPure™ Bacterial DNA Extraction Kit can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP and Southern Blot Analysis.
UOM: 1 * 1 KIT
Product Image-ICNA117022400

DNA extraction kits, tissue DNA extraction kit, MPure™, MP Biomedicals

Catalog Number: (ICNA117022400)
Supplier: MP Biomedicals
Description: MPure™ Tissue DNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction and purification of genomic DNA from a variety of animal tissues, swab samples and blood stains. Nucleic acids extracted and purified from MPure™ Tissue DNA Extraction Kits can be used in a number of downstream applications including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP and Southern Blot Analysis.
UOM: 1 * 1 KIT

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Image Unavailable-40059.

DEAC-dUTP (diethylaminocoumarin-dUTP), lyophilized powder

Catalog Number: (40059.)
Supplier: Biotium
Description: DEAC-dUTP (diethylaminocoumarin-dUTP) can be used to synthesize fluorescently labeled DNA probes for in-situ hybridization, microarray or blotting techniques.
UOM: 1 * 25 Nmo
Product Image-ICNA117022200

DNA extraction, MPure™ Blood DNA extraction kits, MP Biomedicals

Supplier: MP Biomedicals
Description: MPure™ Blood DNA Extraction Kit is used with the MPure-12™ aNAP System for extraction of DNA from 100 to 400 µl mammalian whole blood, peripheral blood mononuclear cells or buffy coat. Nucleic acids extracted from MPure Blood DNA Extraction Kit 200 can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP, Southern Blot Analysis.

Image Unavailable-S4651-72EA

Silane-Prep Slides

Catalog Number: (S4651-72EA)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Silane-prep slides are microscope slides coated with silane (aminoalkysilane) to improve the adhesion of tissues to the slide for immunohistochemistry. The slide includes a 'frosted end' for slide/patient identification. It is not tested for use in microarray assays.
UOM: 1 * 72 items
Product Image-ICNA111103600

GeneClean® PCR clean-up, turbo for PCR kit, MP Biomedicals

Supplier: MP Biomedicals
Description: The GeneClean® Turbo for PCR Kit removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples. DNA fragments purified with this kit are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion and labeling.

Product Image-COBSD7-000

P3 SureLight® molecular biology stain

Catalog Number: (COBSD7-000)
Supplier: Columbia Biosciences
Description: SureLight™ P3 is a proprietary dye can be used in prompt fluorescent detection, microplate applications, flow cytometry and protein microarrays.
UOM: 1 * 5 mg
Image Unavailable-NOVUNB120-11193

Anti-VINC Mouse monoclonal antibody

Catalog Number: (NOVUNB120-11193)
Supplier: Novus Biologicals
Description: The Vinculin Antibody (VIN-11-5) from Novus Biologicals is a mouse monoclonal antibody to Vinculin. This antibody reacts with human, mouse, bovine, chicken. The Vinculin Antibody (VIN-11-5) has been validated for the following applications: Western Blot, Immunocytochemistry / Immunofluorescence, Microarray.
UOM: 1 * 0,1 mL
Image Unavailable-ROCKMB-045

SSC buffer, 20X concentrate, pH 7,0

Catalog Number: (ROCKMB-045)
Supplier: Rockland Immunochemicals
Description: Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, <i>in situ</i> hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.
UOM: 1 * 1 L
Image Unavailable-BOSSBS-1135R-CY5

Anti-SRC Rabbit Polyclonal Antibody (Cy5®)

Catalog Number: (BOSSBS-1135R-CY5)
Supplier: Bioss
Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
UOM: 1 * 100 µl
Image Unavailable-NOVUNB120-10454

Anti-CASP11 Rat monoclonal antibody

Catalog Number: (NOVUNB120-10454)
Supplier: Novus Biologicals
Description: The Caspase-11 Antibody (17D9) from Novus Biologicals is a rat monoclonal antibody to Caspase-11. This antibody reacts with human, mouse. The Caspase-11 Antibody (17D9) has been validated for the following applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry / Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Microarray.
UOM: 1 * 0,1 mL
Image Unavailable-40027.

CF® Dye dCTP conjugates

Supplier: Biotium
Description: CF® dye-dCTP can be used to synthesise probes for in situ hybridisation, microarray, or to synthesise labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.

Image Unavailable-IMMRIR2937

Anti-IgG (H+L) F(ab)'2 Goat Polyclonal Antibody (APC (Allophycocyanin))

Catalog Number: (IMMRIR2937)
Supplier: ImmunoReagents
Description: The ImmunoReagents' Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm. APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability.
UOM: 1 * 0,5 mL
Image Unavailable-40063.

Fluorescein-12-dUTP, lyophilized powder

Catalog Number: (40063.)
Supplier: Biotium
Description: Fluorescein-12-dUTP can be used to synthesize fluorecently labeled DNA probes for in-situ hybridization, microarray or blotting techniques.
UOM: 1 * 25 Nmo
Image Unavailable-BOSSBS-1135R-A680

Anti-SRC Rabbit Polyclonal Antibody (Alexa Fluor® 680)

Catalog Number: (BOSSBS-1135R-A680)
Supplier: Bioss
Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labelling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr --> Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
UOM: 1 * 100 µl
Image Unavailable-BOSSBS-1135R-A750

Anti-SRC Rabbit Polyclonal Antibody (Alexa Fluor® 750)

Catalog Number: (BOSSBS-1135R-A750)
Supplier: Bioss
Description: c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labelling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr --> Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.
UOM: 1 * 100 µl
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