You Searched For: Ethyl+4-chloroacetoacetate

Description: Component of Earle′s balanced salt solution; Component of Hanks′ balanced salt solution; Component of plant cell-culture media and of culture media for microorganisms.; Purification of CAP, catabolite gene activator; In the preparation of spheroplasts from E. coli K38.

Catalog Number: ICNA02191422.5

UOM: 1 * 500 g

Supplier: MP Biomedicals

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Description: Sodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. The 100 mM solution should be diluted 1:100 for most cell culture. The use of sodium pyruvate in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by <i>Bacillus sphaericus</i> has been described. A protocol that uses sodium pyruvate to establish stably transfected human B cell lines has been published. It improves coliform recovery when present in culture medium.

Catalog Number: ICNA091682049

UOM: 1 * 100 mL

Supplier: MP Biomedicals

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Description: VWR International declares that the the growth promotion testing of this product is performed in compliance with ISO 11133:2014 standard “Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media”

Catalog Number: 499319OO

UOM: 1 * 50 mL

Supplier: VWR Chemicals

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Description: Most cells can synthesise non-essential amino acids (NEAA) through glucoaminolysis, glycolysis, or the TCA cycle. When these amino acids concentration levels are low, cells need to consume more glucose and glutamine in the media and produce excess by-products, which might affect cell growth. Adding NEAA into the cell culture medium could mitigate the potential side effects caused by self-production of these non-essential amino acids.

Catalog Number: ICNA091681049

UOM: 1 * 100 mL

Supplier: MP Biomedicals

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Description: This product is a mix of the antibiotics Penicillin (5,000 IU) and Streptomycin (5,000 μg/ mL) in a 50-fold working concentration. Penicillin (Penicillin G) works by inhibiting peptidoglycan synthesis, while Streptomycin inhibits protein synthesis. Penicillin-Streptomycin is effective against Gram-negative and Gram-positive bacteria. This antibiotic is recommended for use in cell culture media at 20 ml/L.

Catalog Number: 30-001-CI

UOM: 1 * 6 items

Supplier: Corning

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Description: Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc.
Tween® 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture selective protein extraction growing of tubercule bacilli, and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent. It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds. Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
Soluble/miscible in water to give a clear yellow solution; miscible with alcohol, dioxane, and ethyl acetate; practically insoluble in liquid paraffin and fixed oils (such as mineral oil).
Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Tween® may need to be warmed to about 40 °C and alternated with portions of hot distilled water while being poured through the filter.
Store at Room Temperature (15-30 °C).

Catalog Number: ICNA02194725.1

UOM: 1 * 100 mL

Supplier: MP Biomedicals

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Description: Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g., a tissue culture flask) under more natural conditions than on a glass slide, as it occurs with a conventional microscope. IM-3 series is engineered and designed to be your ideal solution for fast and reliable routine inspections, with the exclusive, state-of-the-art X-LED8 illumination system.

Catalog Number: OPTKIM3GLASS

UOM: 1 * 1 items

Supplier: OPTIKA

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Description: 293-Free™ transfection reagent has a unique polycationic liposomal formulation and is designed expressly for transfection of HEK 293 cells grown in suspension culture. It is ideal for mammalian protein production. 293-Free™ transfection reagent is provided as a sterile, ready to use solution. The 1 ml size provides enough reagent to transfect a litre of culture.

Catalog Number: 72181-3

UOM: 1 * 1 mL

Supplier: Merck Millipore (Novagen)

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Description: Gentamicin sulphate is a broad-spectrum cell culture antibiotic that is non-toxic to viruses and mammalian cells at anti-bacterial and anti-mycoplasmal concentrations. Its extended stability and the slow development of bacterial resistance allow long-term virus and tissue culture studies. Composed of three closely related compounds: gentamycin C1, C1a, and C2.

Catalog Number: 345815-20ML

UOM: 1 * 20 mL

Supplier: Merck Millipore (Calbiochem‎)

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Description: BTXpress Cytoporation® Media T/T4 is an advanced electroporation buffer designed for use with the BTX AgilePulse MAX™ large volume electroporation system for <i>in vitro</i> delivery of DNA, RNA, oligonucleotides, and siRNA. The low conductivity buffer is specially formulated to minimise heating of solution during large volume electroporation for maximum transfection efficiency and high cell viability. BTXpress Cytoporation® Media T and T4 are sterile filtered from the highest quality non animal, medical grade reagents. Buffer can be directly diluted in complete growth media for post electroporation cell culture.

Catalog Number: BTXI47-0003

UOM: 1 * 500 mL

Supplier: BTX

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Description: HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.

Catalog Number: ICNA091688449

UOM: 1 * 100 mL

Supplier: MP Biomedicals

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Description: These units are ideal for the cold sterilisation of eg. cell culture media and protein solutions. PES 'high-flow' filter membrane for high throughput and low protein binding.

Catalog Number: SARS83.1822

UOM: 1 * 12 items

Supplier: SARSTEDT

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Description: Storage: Room Temperature, desiccate
Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.
Potassium phosphate monobasic is typically used as a component for a wide variety of media used in the culture of microorganisms. It is widely used in molecular biology, biochemistry, and chromatography. It is a component in phosphate buffered saline (PBS). In addition to helping maintain pH, it supplies essential phosphate.

Catalog Number: ICNA0219472791

UOM: 1 * 1 kg

Supplier: MP Biomedicals

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Description: Poloxamer 188 (P188) is a non-ionic amphiphilic copolymer. It comprises a central chain of hydrophobic polyoxypropylene flanked at ends by hydrophilic polyoxyethylene.

Catalog Number: SIALP5556-100ML

UOM: 1 * 100 mL

Supplier: Merck

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Description: Platform shaker with three dimensional gyratory motion, designed for homogeneous mixing, ideal for distribution of cells or solutes, preparing slides and electrophoresis gels, culture plates and Erlenmeyer flasks. Polymax 2040 is suitable for large vessels and culture plates. Units have analogue dial controls for adjusting wave frequency and time.

Catalog Number: 444-1458

UOM: 1 * 1 items

Supplier: Heidolph Instruments GmbH & Co.KG

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Description: The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Catalog Number: PIER75710

UOM: 1 * 1 SET

Supplier: Thermo Fisher Scientific

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