You Searched For: IPTG+(Isopropyl-\\u03B2-D-thiogalactopyranoside)

Catalog Number: (PIER34060)

Supplier: Thermo Fisher Scientific

Description: Thermo Scientific Pierce IPTG is isopropyl-β-D-thiogalactopyranoside for use with beta-galactosidase substrates such as X-Gal to detect expression of vectors, plasmids, or DNA fragments that contain the reporter gene.

UOM: 1 * 1 g

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Supplier: Merck

Description: IPTG (isopropyl-β-D-thiogalactopyranoside), Sigma-Aldrich®

Catalog Number: (MDTC46-102-RF)

Supplier: Corning

Description: IPTG (isopropylthiogalactoside) is an inducer of the lac operon in bacteria, which is frequently used in cloning as a component of a recombinant plasmid.

UOM: 1 * 1 g

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Supplier: MP Biomedicals

Description: Cell culture media component for use in molecular genetics. β-Galactoside inducer.

Catalog Number: (BE-312)

Supplier: G-Biosciences

Description: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).

UOM: 1 * 1 KIT

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Supplier: Apollo Scientific

Description: 4-Methylphenyl-beta-D-thiogalactopyranoside 99% min

Catalog Number: (APOSBIB6257-1G)

Supplier: Apollo Scientific

Description: Phenylethyl-β-D-thiogalactopyranoside

UOM: 1 * 1 g

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Supplier: Apollo Scientific

Description: 4-Methylphenyl-2,3,4,6-tetra-O-acetyl-β-D-thiogalactopyranoside 99% min

Supplier: Apollo Scientific

Description: Salmon-Gal is an alternative chromogenic substrate for b-D-Galactisidase. Salmon-Gal is used in conjunctionwith IPTG for detection of b-Galactosidase activityin bacterial colonies.

Catalog Number: (437132J)

Supplier: VWR Chemicals

Description: X-Gal is a chromogenic substrate for ß-Galactosidase that forms an intense blue precipitate. Used in conjunction with IPTG in a colorimetric assay, X-Gal allows differentiation of recombinants from non recombinants in cloning experiments with vectors containing the lacZ gene. It is also used in microbiology to detect microorganisms which have ß-Galactosidase activity.

UOM: 1 * 1 g

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Supplier: Apollo Scientific

Description: X-Gal, a chromogenic substrate of beta-Galactosidase. Used in conjunction with IPTG for detection of beta-Galactosidase activity in bacterial colonies in a colorimetric assay.

Catalog Number: (EDVO221)

Supplier: EDVOTEK

Description: In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the <i>lacZ</i> gene. IPTG is not required in this experiment since pGAL contains the complete <i>lacZ</i> gene.

UOM: 1 * 1 items

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Supplier: G-Biosciences

Description: X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

Supplier: VWR Chemicals

Description: Isopropyl myristate ≥98%, GPR RECTAPUR®

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Supplier: VWR Chemicals

Description: Isopropyl acetate ≥99%, GPR RECTAPUR®

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Supplier: Merck

Description: 5-Isopropyl-2-methylphenol, Sigma-Aldrich®

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