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Taq CORE kits, MP Biomedicals

Supplier: MP Biomedicals
Description: The Taq CORE kits are designed for the amplification of specific regions of DNA using PCR. The PCR CORE Kits - Direct Loading provide <i>Taq</i> DNA Polymerase, PCR Direct Loading buffers and dNTPs necessary for amplification and subsequent loading of samples on an agarose gel.

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Tritirachium album proteinase K, MP Biomedicals

Supplier: MP Biomedicals
Description: Proteinase K is a highly active stable endopeptidase with a broad spectrum of action was isolated by E. Merk's Darmstadt Biochemical Research Department in 1970 from a culture filtrate of the fungus, Tritirachium album Limber. This fungus is able to grow on Keratin (e.g., wool, horn particles) as the sole source of carbon and nitrogen. The isolated protease was, therefore, given the K designation.
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0,1 to 0,5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

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PCR beads

Catalog Number: (EDVO625)
Supplier: EDVOTEK
Description: Each PCR Bead contains <i>Taq</i> DNA polymerase, <i>Taq</i> DNA polymerase buffer, dNTP mixture and MgCl₂. Pack contains sufficient for 25 reactions.
UOM: 1 * 1 items
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Arginine-C™, sequencing grade

Catalog Number: (786-11)
Supplier: G-Biosciences
Description: SG-Arginine-C™ endopeptidase (Clostripain, from <i>C. histolyticum</i>) specifically hydrolyses the carboxy peptide bond of arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilise enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.
UOM: 1 * 2 items
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CAPS (3-(cyclohexylamino)propanesulphonic acid), white powder

Supplier: MP Biomedicals
Description: CAPS is a zwitterionic biological buffer which has minimal reactivity with enzymes or proteins and minimal salt effects. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8,5.

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Controlled Protein-Protein Crosslinking Kit, Pierce™

Catalog Number: (PIER23456)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.
UOM: 1 * 1 KIT
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Glycerine ≥99.5%, Ultrapure

Supplier: MP Biomedicals
Description: Mercury: <10ppm Lead: <5 ppm
Iron: <5 ppm Zinc: <2 ppm
Used in the concentration and storage of enzymes. Also prevents back-diffusion and protein samples into the buffer.

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Phusion® Green High-Fidelity DNA Polymerase

Supplier: FINNZYMES REAGENTS
Description: Phusion® Green High-Fidelity DNA Polymerase is a combination of Phusion® DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

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PCR clean-up kit, Axygen® AxyPrep™ Mag

Supplier: Corning
Description: The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

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Capsule filters, Polycap™ SPF, Whatman™

Supplier: Whatman products (Cytiva)
Description: These Polycap™ SPF capsule filters are optimised for prefiltration applications. They are typically used upstream of a Polycap™ AS or Polycap™ TC capsule during enzyme, nutrient media or buffer filtration.

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Glycerine ≥99%, clear colourless viscous liquid cell culture reagent

Supplier: MP Biomedicals
Description: Storage: Store at Room Temperature (15 - 30 °C)
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. Glycerol is used in the concentration and storage of enzymes. Also prevents back-diffusion and protein samples into the buffer. Ideal for use as a cryoprotectant.

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Phusion® Green hot start II High-fidelity DNA polymerase

Supplier: FINNZYMES REAGENTS
Description: Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

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PCR master mix, Taq-&LOAD™, MP Biomedicals

Supplier: MP Biomedicals
Description: Taq-&LOAD™ PCR Master Mix is an optimised 5X reaction mix for PCR containing recombinant <i>Taq</i> DNA polymerase, dNTPs, MgCl₂, and incubation and loading buffers. Also included are a compound increasing the density of the sample and a red/purple dye.

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GMBS (N-Succinimidyl 4-maleimidobutyrate) ≥98% (by HPLC), Sigma-Aldrich®

Supplier: Merck
Description: A heterobifunctional cross-linking reagent with amine and sulfhydryl reactivity. Typically, coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). Second coupling specific for molecules containing free sulfhydryl by thioether linkage buffered at pH 6.8 (6.5-7.0). Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates. Provides a 7-atom linker.

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4-Nitrophenyl phosphate disodium salt (pNNP) substrate system, liquid, Sigma-Aldrich®

Catalog Number: (SIALN7653-100ML)
Supplier: Merck
Description: The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.
UOM: 1 * 100 mL
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Phusion® green hot start II high-fidelity PCR master mix

Supplier: FINNZYMES REAGENTS
Description: Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

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The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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