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Product Image-F-530L

Phusion® High-Fidelity DNA Polymerase

Supplier: FINNZYMES REAGENTS
Description: Phusion® High Fidelity DNA Polymerase generates PCR products with an accuracy and speed unattainable with a single enzyme, even on the most difficult templates. The structure and characteristics of Phusion® High Fidelity DNA Polymerase make it a superior choice for cloning, and sets the standard for PCR performance with an error rate 50-fold lower than that of <i>Taq</i> DNA polymerase, and 6-fold lower than that of <i>Pfu</i> DNA polymerase. In addition, Phusion® High Fidelity DNA Polymerase possesses processivity 10-fold greater than <i>Pfu </i>DNA polymerase.

SDS

Product Image-F-170S

Tissue direct PCR master mix, Phire™

Supplier: FINNZYMES REAGENTS
Description: Phire™ Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The master mix containing Phire™ Hot Start II DNA polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors, such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle).

Product Image-EP1713

DreamTaq™ Hot Start DNA polymerase

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq™ Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.

Product Image-AATB17316

ReadiLink™ iFluor® 647 FISH Fluorescence Imaging Kit

Catalog Number: (AATB17316)
Supplier: AAT BIOQUEST
Description: The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 647-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).
UOM: 1 * 25 Tests
Product Image-786-449

Taq DNA polymerase

Supplier: G-Biosciences
Description: <i>Taq</i> DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile <i>Thermus aquaticus</i>. The molecular weight of the recombinant protein is 94 kD. The <i>Taq</i> Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this <i>Taq</i> Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. <i>Taq</i> DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Product Image-K1642

Maxima™ First strand cDNA synthesis kit

Supplier: Thermo Fisher Scientific
Description: The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Product Image-D1196-01

Tissue DNA kit, genomic DNA isolation from tissue, E-Z 96®

Supplier: OMEGA BIO-TEK
Description: By adapting HiBind® technology with 96-well plates, the E-Z 96® Tissue DNA kit provides a high throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridisation techniques.

SDS

Product Image-K1632

RevertAid™ H minus first strand cDNA synthesis kits

Supplier: Thermo Fisher Scientific
Description: RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Product Image-710-0672

Real-Time PCR kit for detection of Cronobacter sakazakii

Supplier: Q BIOANALYTIC
Description: Cronobacter sakazakii (formerly Enterobacter sakazakii) is found naturally in the environment and can survive in dry conditions. It occurs in dry foods, such as powdered infant formula, milk powder and starches as well as in teas. Especially young infants are at high risk developing severe meningitis, if infected. All common Real-Time PCR block cyclers are usable for the test. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons. The test can also be used for the confirmation of bacterial colonies. The Real-Time PCR Test allows the detection of the species with high accuracy and is more precise than, cultural methods often are.

Product Image-28-9006-54

illustra™ Hot Start mix, Ready-To-Go™

Supplier: Cytiva
Description: illustra™ Hot Start Mix, Ready-To-Go™ is a pre-formulated and pre-dispensed, freeze-dried PCR reagent mix, including high quality PuReTaq™ DNA polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 or 0,2 ml tubes that are compatible with most thermal cyclers.

Product Image-F-541

Phusion® site-directed mutagenesis kit

Catalog Number: (F-541)
Supplier: FINNZYMES REAGENTS
Description: Phusion® Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA.
UOM: 1 * 1 KIT
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First strand cDNA synthesis kit

Catalog Number: (K1612)
Supplier: Thermo Fisher Scientific
Description: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.
UOM: 1 * 1 KIT
Product Image-K1072

DreamTaq™ PCR master mix (2X)

Supplier: Thermo Fisher Scientific
Description: DreamTaq™ PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq™ buffer, MgCl₂ and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. DreamTaq™ DNA polymerase is an enhanced <i>Taq</i> polymerase optimised for high throughput PCR applications. It ensures robust amplification of PCR products up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Product Image-460-0261

Digital dry block heaters

Supplier: IKA
Description: Digital block heaters which hold from one up to four aluminum blocks, provide precise temperature control in small vessels. The PT1000 temperature sensor allows temperature control directly in the sample vessel. It is used with PCR tubes, PCR strips, Greiner tubes, microplates and cuvettes. The block heaters are ideal for melting and boiling point determination, enzyme reactions, incubation and activation of cultures, immunoassays, DNA denaturation, culture media tests, coagulation tests, blood/urea nitrogen determinations and <i>in situ</i> hybridisations.

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Master mixes, DreamTaq™ Hot Start PCR master mix (2X)

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready to use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq™ buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. The green master mix includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

Product Image-29-3430-57

PCR clean-up reagent, Sera-Mag™ select

Supplier: Cytiva
Description: Sera-Mag Select PCR clean-up, size selection reagent combines the exceptional binding characteristics of Sera-Mag carboxyl speedbeads with an optimised binding solution in a ready to use formulation. The DNA size range isolated from the process can be tailored to suit the user’s end requirements by adjusting the amount of reagent that is mixed with a fixed volume of sample.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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