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PCR master mix (2X)

Supplier: Thermo Fisher Scientific
Description: PCR Master Mix is a 2X concentrated solution of <i>Taq</i> DNA polymerase, dNTPs and all other components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimised for efficient and reproducible PCR.

Certificates

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RT-PCR kits, Verso™

Supplier: Thermo Fisher Scientific
Description: The Verso™ RT-PCR system combines a reverse transcription enzyme, improved priming options and optimised buffers to generate high yield and full length cDNA. With a variety of kit options for both 1- and 2-step RT-PCR reactions, Verso™ provides optimum flexibility and sensitivity in an easy to use format.

Product Image-BIOPFS-SP0047

Food safe kits for identification of pathogenic microorganisms

Supplier: BIOPREMIER
Description: Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of a specific pathogenic bacteria. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target microorganism. PCR is a method used to amplify a specific DNA sequence which is typically amplified in a reaction containing a thermostable DNA polymerase, nucleotides, and primers complementary to the target sequence. When this solution is heated, the DNA molecule denatures, separating into two strands. As the solution cools, the primers anneal to the target sequences in the separated DNA strands and the DNA polymerase synthesises a new strand by extending the primers with nucleotides, creating a copy of the DNA sequence (amplicons). When repeated, this cycle of denaturing, annealing, and extending exponentially increases the number of target amplicons. In Real-Time PCR, specific fluorescent probes are used to detect the amplified DNA by hybridising with amplicons. These probes are linked to a fluorophore on one end and a quencher that suppresses fluorescence on the other. If the target sequence is present during the PCR, amplification occurs and the probe is degraded, resulting in fluorescence increase. Fluorescence is measured by a detector and the associated software plots the fluorescence intensity versus number of cycles, allowing the determination of the presence or absence of the target organism.

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Phusion® High-Fidelity PCR Master Mix with GC Buffer

Supplier: FINNZYMES REAGENTS
Description: Phusion® High-Fidelity PCR Master Mix with GC Buffer is a convenient 2X mix containing Phusion® DNA polymerase, nucleotides and optimised GC buffer including MgCl₂. Only template and primers need to be added by the user.

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KOD Xtreme™ Hot Start DNA polymerase

Catalog Number: (71975-3)
Supplier: Merck Millipore (Novagen)
Description: KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.
UOM: 1 * 200 AU
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LAL chromogenic endotoxin assay kit, ToxinSensor™

Supplier: Genscript
Description: ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit is designed to be a quantitative <i>In vitro</i> end-point endotoxin test for human and animal parenteral drugs, biological products, and medical devices.

SDS

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DreamTaq™ DNA polymerase

Supplier: Thermo Fisher Scientific
Description: DreamTaq™ DNA Polymerase is an enhanced <i>Taq</i> DNA polymerase optimised for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional <i>Taq</i> DNA polymerase. DreamTaq™ DNA Polymerase uses the same reaction set-up and cycling conditions as conventional <i>Taq</i> DNA polymerase. Extensive optimisation of reaction conditions is not required. The enzyme is inhibited by dUTP but can incorporate modified nucleotides. Applications include routine PCR amplification of DNA fragments up to 6 kb from genomic DNA and up to 20 kb from viral DNA, RT-PCR, and generation of PCR products for TA cloning.

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ToxinSensor™ single tests kit with standard

Supplier: Genscript
Description: ToxinSensor™ single tests kit with standard is designed to be a qualitative In Vitro end-point endotoxin test for human and animal parenteral drugs, biological products, and medical devices.

Certificates

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Phusion® High-Fidelity DNA Polymerase

Supplier: FINNZYMES REAGENTS
Description: Phusion® High Fidelity DNA Polymerase generates PCR products with an accuracy and speed unattainable with a single enzyme, even on the most difficult templates. The structure and characteristics of Phusion® High Fidelity DNA Polymerase make it a superior choice for cloning, and sets the standard for PCR performance with an error rate 50-fold lower than that of <i>Taq</i> DNA polymerase, and 6-fold lower than that of <i>Pfu</i> DNA polymerase. In addition, Phusion® High Fidelity DNA Polymerase possesses processivity 10-fold greater than <i>Pfu </i>DNA polymerase.

SDS

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ReadiLink™ iFluor® 555 FISH Fluorescence Imaging Kit

Catalog Number: (AATB17313)
Supplier: AAT BIOQUEST
Description: The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 555-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).
UOM: 1 * 25 Tests
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Taq DNA polymerase

Supplier: G-Biosciences
Description: <i>Taq</i> DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile <i>Thermus aquaticus</i>. The molecular weight of the recombinant protein is 94 kD. The <i>Taq</i> Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this <i>Taq</i> Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. <i>Taq</i> DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

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Mag-Bind® Plant DNA DS 96 kit

Supplier: OMEGA BIO-TEK
Description: The Mag-Bind® Plant DNA DS 96 Kit is designed for difficult samples (DS) which are hard to lyse or contain high amounts of polysaccharides and polyphenols. The system uses a CTAB-based lysis buffer which does not require organic solvents. The proprietary binding system prevents inhibitors from binding to the magnetic beads. The wash buffer system further removes polysaccharides, phenolic compounds and enzyme inhibitors from plant tissue lysate. An optional rebinding step is included with the kit but most samples do not require the additional treatment.

SDS

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Mag-Bind® Plant DNA plus kit

Supplier: OMEGA BIO-TEK
Description: The Mag-Bind® Plant DNA 96 plus kit allows rapid and reliable isolation of high-quality genomic DNA from a wide variety of difficult to lyse and high polysaccharide plant species and tissues. The system utilises a CTAB-based lysis buffer without the need for a chloroform or other organic solvent steps. The proprietary wash buffer systems eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. The Mag-Bind® Plant DNA plus kit also provides greater flexibility than silica plate based systems as partial plates can be used and can be scaled up to a 24-well format with the appropriate magnetic stand. The streamlined protocol can be easily adapted on most robotic liquid handling platforms. Purified DNA is suitable for PCR, qPCR, restriction digestion, hybridisation applications, as well as more demanding applications, such as RAPD.

SDS

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ReadiLink™ iFluor® 647 FISH Fluorescence Imaging Kit

Catalog Number: (AATB17316)
Supplier: AAT BIOQUEST
Description: The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 647-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).
UOM: 1 * 25 Tests
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Verso™ qRT-PCR Kits

Supplier: Thermo Fisher Scientific
Description: The Verso™ qRT-PCR system combines the Verso™ RT enzyme with ABsolute™ Blue qPCR master mix to provide maximum convenience and versatility. Available in a 1- or 2-step format, as well as multiple pack sizes, to suit all application and throughput requirements.

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ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit

Catalog Number: (AATB17310)
Supplier: AAT BIOQUEST
Description: The kit provides Taq DNA polymerase enzyme, which incorporates iFluor® 488-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).
UOM: 1 * 25 Tests
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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