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E.Z.N.A.® Endo-Free plasmid mini kit II

Supplier: OMEGA BIO-TEK
Description: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

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Bacterial DNA kit, E.Z.N.A.®

Supplier: OMEGA BIO-TEK
Description: The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

SDS Certificates

Product Image-D6943-02

E.Z.N.A.® Plasmid Mini kit I

Supplier: OMEGA BIO-TEK
Description: The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind® Mini Column technology into three quick steps: Bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing. E.Z.N.A.® Plasmid Mini Kit I is available in either V-spin or Q-spin column format. V-spin column formats have an attached lid while Q-spin columns do not. Both versions can be used for centrifugation or vacuum protocols.

SDS Certificates

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E.Z.N.A.® Plant RNA kit

Supplier: OMEGA BIO-TEK
Description: E.Z.N.A.® Plant RNA kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. This kit provides a homogeniser column for filtration and homogenisation of viscous plant cell lysate by centrifugation in combination with the HiBind® RNA spin column for RNA purification. All the contaminants including polysaccharides and phenolic compounds are effectively removed. Purified RNA can be used for most downstream applications such as RT-PCR, northern blot analysis, differential display, and poly(A)+ RNA selection.

SDS Certificates

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T4 UvsX DNA Recombinase

Supplier: G-Biosciences
Description: T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes. The protein forms a right-handed nucleoprotein complex on single ssDNA called the presynaptic filament that can search for homology in duplex DNA and pair the recombining DNA molecules to form a DNA joint molecule. This process is aided by ssDNA binding proteins and recombination mediators. The filament is then elongated by DNA polymerase with the newly synthesised strand displacing the old strand. The newly synthesised duplex DNA can then act as a template for the next cycle to achieve exponential amplification of dsDNA. <i>E.coli</i> cells expressing recombinant T4 UvsX gene.

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MicroElute® Cycle-Pure & Gel Extraction Kit, Omega Bio-tek

Supplier: OMEGA BIO-TEK
Description: MicroElute® Clean Up system, designed for rapid DNA clean up.

SDS

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Dideoxyuridine triphosphates (ddUTPs)

Supplier: ENZO LIFE SCIENCES
Description: These ddUTPs are used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. ddUTP also serves as a substrate for <i>E. coli</i> DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase.

Product Image-MILFLSKP09624

Montage® Plasmid Miniprep 96 Kits

Supplier: Merck
Description: Plasmid Miniprep 96 kit is a quick and easy-to-use kit that produces clean and reproducible DNA in half a time than traditional methods.

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RNA isolation, total RNA kit II, E.Z.N.A.®

Supplier: OMEGA BIO-TEK
Description: The E.Z.N.A.® Total RNA kit II is designed for isolating total cellular RNA from tissues rich fibrous and fatty tissues such as skeletal muscle, heart, brain and adipose tissues. Compared to other standard silica-column procedures, the E.Z.N.A.® Total RNA kit II provides higher yield and better quality of RNA from all types of tissue. This kit combines phenol/guanidine-base lysis and the silica membrane purification of RNA technology to provide a rapid and easy method of the isolation of total RNA from any tissue sample. RNA purified using the E.Z.N.A.® Total RNA method is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and<i> in vitro</i> translation.

SDS Certificates

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E.Z.N.A.® Plasmid Mini kit II, Q-spin (without lid)

Supplier: OMEGA BIO-TEK
Description: This second generation plasmid miniprep kit yields 40 to 70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 ml to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.

SDS Certificates

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E.Z.N.A.® Total RNA Kit I

Supplier: OMEGA BIO-TEK
Description: Isolate total RNA from cells or soft tissues using spin column.

Product Image-ENZOENZNUC1130025

Digoxigenin-dUTP, alkali-stable

Catalog Number: (ENZOENZNUC1130025)
Supplier: ENZO LIFE SCIENCES
Description: Digoxigenin-labeled nucleotide suitable for non-radioactive labeling of DNA probes.
UOM: 1 * 25 Nmo
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Formalin-fixed, paraffin-embedded (FFPE) tissue RNA kits, E.Z.N.A®

Supplier: OMEGA BIO-TEK
Description: Selected protease digestion releases microgram amounts of RNA from FFPE samples. The purified nucleic acids, although highly fragmented, are suitable for a variety of downstream genomic and gene expression analyses. E.Z.N.A® spin column-based kits are ideal for low throughput applications while Mag-Bind® magnetic beads-based kits are designed specifically for high throughput users with automation capability.

SDS Certificates

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Acridine orange (chlorid salt), hydrate ≥98% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Acridine orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine orange is also used to analyse autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.

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TriLink® 2'-Deoxyadenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies
Description: This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

New Product

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PCR decontamination kit

Catalog Number: (ENZOENZKIT1370100)
Supplier: ENZO LIFE SCIENCES
Description: Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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