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Product Image-PIER22312

Sulpho-MBS (3-Maleimidobenzoyl-N-hydroxysulphosuccinimide ester sodium salt), Pierce™

Catalog Number: (PIER22312)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-MBS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short aromatic spacer arm (7.3 angstroms).
UOM: 1 * 50 mg
Product Image-PIER26175

Sulpho-SDAD (Sulpho-NHS-SS-Diazirine) (Sulphosuccinimidyl 2-[(4,4′-azipentanamido)ethyl]-1,3′-dithiopropionate], Pierce™

Catalog Number: (PIER26175)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.
UOM: 1 * 50 mg
Image Unavailable-PIERA39269

Sulpho-NHS (N-Hydroxysulphosuccinimide sodium salt), No-Weigh™ Format, Pierce™

Catalog Number: (PIERA39269)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-NHS, No-Weigh Format is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.
UOM: 1 * 20 mg
Product Image-PIER26174

Sulpho-LC-SDA (Sulpho-NHS-LC-Diazirine) (Sulphosuccinimidyl 6-(4,4′-azipentanamido)hexanoate), Pierce™

Catalog Number: (PIER26174)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.
UOM: 1 * 50 mg
Image Unavailable-SIAL803235-50MG

Sulpho-EMCS (N-(ε-maleimidocaproyloxy) sulphosuccinimide ester), Sigma-Aldrich®

Catalog Number: (SIAL803235-50MG)
Supplier: Merck
Description: EMCS and its water-soluble analog Sulfo-EMCS are heterobifunctional cross-linkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugation experiments involving these cross-linkers are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.
UOM: 1 * 50 mg
Product Image-PIER21111

Sulpho-KMUS, Pierce™

Catalog Number: (PIER21111)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-KMUS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (16.3 angstroms).
UOM: 1 * 50 mg
Product Image-PIER21335

Sulpho-NHS-LC-Biotin, EZ-Link™, Pierce™

Catalog Number: (PIER21335)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
UOM: 1 * 100 mg

SDS


Image Unavailable-PIERPG82075

Sulpho-NHS-LC-Biotin, Premium Grade, Pierce™

Catalog Number: (PIERPG82075)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Premium Grade Sulfo-NHS-LC-Biotin is our highest quality formulation of this popular amine-reactive biotinylation reagent, specially characterized for applications where product integrity and risk minimization are paramount.
UOM: 1 * 100 mg
Product Image-PIER21441

Amine reactive biotinylation reagents, EZ-Link™

Supplier: Thermo Fisher Scientific
Description: The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Product Image-PIER22327

Sulpho-SIAB (Sulphosuccinimidyl (4-iodoacetyl)aminobenzoate), Pierce™

Catalog Number: (PIER22327)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-SIAB a water-soluble, mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via sulfo-N-hydroxysuccinimide (sulfo-NHS) ester and iodoacetyl reactive groups.
UOM: 1 * 50 mg
Image Unavailable-SIAL803642-50MG

LC-SPDP (Succinimidyl 6-[3-(2-pyridylthio)propionamidohexanoate) ≥95%, Sigma-Aldrich®

Catalog Number: (SIAL803642-50MG)
Supplier: Merck
Description: The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents – see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures.
UOM: 1 * 50 mg
Image Unavailable-PIERA39257

Sulpho-NHS-LC-Biotin, EZ-Link™ No-Weigh™ Format, Pierce™

Catalog Number: (PIERA39257)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
UOM: 1 * 10 mg

Certificates


Image Unavailable-SIAL803332-50MG

Sulpho-SANPAH (Sulphosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate), Sigma-Aldrich®

Catalog Number: (SIAL803332-50MG)
Supplier: Merck
Description: ANB-NOS and Sulfo-SANPAH are heterobifunctional crosslinkers that contain an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide. NHS esters react efficiently with primary amino groups (-NH2) in pH 7-9 buffers to form stable amide bonds. When exposed to UV light nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines).
UOM: 1 * 50 mg
Image Unavailable-SIAL56485-1G

Sulpho-NHS (N-Hydroxysulphosuccinimide sodium salt), Sigma-Aldrich®

Supplier: Merck
Description: N-Hydroxysulfosuccinimide sodium salt is a water-soluble sulfonated analog of N-hydroxysuccinimide. It can undergo a coupling reaction with carboxylic acids in the presence of carbodiimides such as 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC) to form hydrophilic N-hydroxysulfosuccinimide active esters. These esters are useful in protein cross-linking experiments.

Product Image-PIER22589

Sulpho-SANPAH (Sulphosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate), Pierce™

Catalog Number: (PIER22589)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.
UOM: 1 * 50 mg
Product Image-PIER21566

Sulpho-EGS (Ethylene glycol bis(sulphosuccinimidyl succinate)), Pierce™

Catalog Number: (PIER21566)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific Pierce Sulfo-EGS is a water-soluble crosslinker that contains amine-reactive sulfo-NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.
UOM: 1 * 50 mg
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
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The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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