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Proteinase K solution

Supplier: PanReac AppliChem
Description: Proteinase K is used to destruct proteins in cell lysates (tissue, cultured cells) and to liberate nucleic acids, since it very effectively digests DNases and RNases.

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L-alanyl L-glutamine, SG-200, HyClone™

Catalog Number: (HYCLSH30590.01)
Supplier: HyClone products (Cytiva)
Description: SG-200 solution is a stable dipeptide of L-alanyl L-glutamine.
UOM: 1 * 100 mL

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Trypsin

Supplier: Biowest
Description: Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

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Protease inhibitor cocktails, ProteaseArrest™

Supplier: G-Biosciences
Description: The ProteaseArrest™ family of protease inhibitor cocktails are supplied as convenient, ready-to-use, liquid format cocktails that offer immediate >95% inhibition of harmful proteases. The 100X concentrated solution outperforms other similar protease inhibitor cocktails (including tablet formats) by offering instant inhibition, allowing delivery of optimised concentrations, demonstrating higher inhibition levels, and providing a greater ease of use. Also available as single use OneQuant™ ProteaseArrest™ aliquots.

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MEM non essential amino acids 100X

Catalog Number: (X0557-100)
Supplier: Biowest
Description: Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.
UOM: 1 * 100 mL

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Phenol red sodium salt, powder, Bioreagent cell culture reagent, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
Description: Phenol Red Sodium Salt (PRSS) is a water-soluble anionic fluorescent dye, that possesses an extra Na ion attached with the SO3 group compared to phenol red. It is intended for use in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6,4 or below and a red color at a pH of 8,2 and above.

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Precellys Multi-Tissue Dissociation Kit

Catalog Number: (BERTD05717.20EA)
Supplier: Bertin Instruments
Description: <B>Optimise tissue dissociation:</B> The Precellys Multi-Tissue Dissociation Kit developed by Bertin Technologies is an innovative and patented solution designed for the obtention of viable single cells from biological samples for research purposes. It enables the obtention of high-quality single cells from complex tissues such as mouse spleen, liver, lung, heart, and thymus and opens the door to downstream single-cell analysis, such as flow cytometry or NGS.
UOM: 1 * 1 KIT

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VWR®, Bottle-Top Vacuum Filtration Systems, PES

Supplier: VWR Collection
Description: PS funnel and collection reservoir. Systems for vacuum filtration of aqueous solutions including cell culture media, buffers or other biological fluids.

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Natural product library, Screen-Well®

Supplier: ENZO LIFE SCIENCES
Description: Natural products are an unsurpassed source of chemical diversity and an ideal starting point for any screening program for pharmacologically active small molecules. Historically, natural products have been the most successful source of new drugs. The Screen-Well® natural product library offers over 500 isolated natural compounds of known structure and includes many compounds from ethnopharmacology. Compounds are provided in solution in DMSO. Individual compounds or subsets can be resupplied in gram quantities.

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Halt™ Protease and Phosphatase Inhibitor Cocktail

Supplier: Thermo Fisher Scientific
Description: Halt protease and phosphatase inhibitor cocktail provides the convenience of a single solution with full sample protection. This broad-spectrum inhibitor cocktail is specifically optimised to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases.

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Cryo solution, Provitro

Catalog Number: (PRTV2040101)
Supplier: PROVITRO
Description: In a serum-free cell culture system, it is very important to cryopreserve cells also in a serum-free freeze medium and not in a serum containing medium. Using an ordinary serum containing freeze medium, the serum affects the cell culture system over a long period. This influence can only be removed by dilution during a large number of passages after seeding.
UOM: 1 * 125 mL

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VWR® VisiScope® IT417 PH Inverted Microscopes

Supplier: VWR Collection
Description: This brightfield, phase contrast microscope is the ideal solution for fast and reliable routine inspections, especially in <i>in vivo</i> cell culture applications.

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Human M2 macrophages (monocyte derived) from peripheral blood

Supplier: Charles River Laboratories Cell Solutions
Description: Human peripheral blood M2 macrophages are derived from purified monocytes. CD14<sup>+</sup> monocytes were positively selected via immunomagnetic beads and were cultured in the presence of M-CSF for 10 days. The M2 phenotyping was confirmed by morphology and the expression of specific biomarkers which monitored by flow cytometry. The cells were harvested after 10 days of <i>in vitro </i>culture.

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Imager assay package, THUNDER

Catalog Number: (630-3396)
Supplier: LEICA MICROSYSTEMS
Description: THUNDER imager assay is an innovative solution for advanced 3-D cell culture assays, whether for analysing stem cells, spheroids or organoids.
UOM: 1 * 1 SET
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Protease inhibitor cocktail, Halt

Supplier: Thermo Fisher Scientific
Description: Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

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Inverted routine microscopes with fixed stage and Fluo package options, DM IL LED

Supplier: LEICA MICROSYSTEMS
Description: The Leica DM IL is a fluorescence-ready digital imaging solution designed for use in a variety of laboratory settings including cell culture, micromanipulation, immuno staining and routine live imaging.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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