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Density gradient medium, OptiPrep™

Catalog Number: (D1556-250ML)

Supplier: SIGMA-ALDRICH MICROSCOPY

Description: Density gradient medium is suitable for cell and subcellular organelle isolation, virus purification, and isolation of lipoproteins and macromolecules.

UOM: 1 * 250 mL

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VWR® Ficoll® 400 Biotechnology Grade

Supplier: VWR Chemicals

Description: Ficoll® 400 is a neutral, hydrophilic polymer of sucrose, which readily dissolves in aqueous solutions.

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VWR®, Lymphocyte separation media

Supplier: VWR Chemicals

Description: Lymphocyte separation media is a density gradient medium for the isolation of mononuclear cells from peripheral blood, cord blood and bone marrow.

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Percoll®, Siam

Supplier: SIGMA-ALDRICH MICROSCOPY

Description: Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. It consists of colloidal silica particles of 15 – 30 nm diameter (23% w/w in water), coated with polyvinylpyrrolidone (PVP).

Lymphocyte separation media, LymphoSep™

Supplier: Biowest

Description: Lymphosep is a standardised, high quality medium designed for simple and rapid isolation of PBMCs from treated whole blood. Lymphosep, like Ficoll™, has the optimised density 1,077 g/ml for lymphocyte separation to guarantee best and reliable results for your preparations.

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VWR®, Trypsin-EDTA solutions

Supplier: VWR Chemicals

Description: VWR® Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.

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Phytohaemagglutinin-M (PHA-M)

Catalog Number: (L3010-005)

Supplier: Biowest

Description: Phytohaemagglutinin is a lectin extracted from red kidney beans (<i>Phaseolus vulgaris</i>). PHA-M is the mucoprotein form and is a crude extract used for the stimulation of cell proliferation in lymphocyte culture. PHA-M also has a powerful erythroagglutinating property and it was originally used for separating leukocytes from whole blood.

UOM: 1 * 5 mL

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Halt™ Protease and Phosphatase Inhibitor Cocktail

Supplier: Thermo Fisher Scientific

Description: Halt protease and phosphatase inhibitor cocktail provides the convenience of a single solution with full sample protection. This broad-spectrum inhibitor cocktail is specifically optimised to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases.