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Catalog Number: (BSENR-1654-100)
Supplier: Biosensis
Description: mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). The mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties (1-3). The resulting monomeric protein is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which is monomeric and has an excitation maximum at 587 nm and and emission maximum at 610 nm (4).
UOM: 1 * 100 µG


Supplier: Thermo Fisher Scientific
Description: Methyl green zinc chloride salt, pure, certified

SDS

Catalog Number: (ROCK600-401-P16S)
Supplier: Rockland Immunochemicals
Description: mCherry antibody is ideal for western blotting. Fluorescent proteins such as Discosoma Red Fluorescent Protein (and its varients), and GFP are widely used in research practice. Both commonly serve as a markers for gene expression and protein localization. DsRed was isolated from sea anemone Discosoma sp. mushroom and GFP is originated from Aequorea victoria jellyfish. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional steps in the chromophore maturation and obligates tetrameric structure. All mCherry antibodies have been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein.
UOM: 1 * 25 µl


Supplier: VWR Chemicals
Description: For various microbiological applications.
Catalog Number: (3076.0025)
Supplier: VWR Chemicals
Description: Malachite green oxalate for microscopy
UOM: 1 * 25 g

Supplier: Merck
Description: Dehydrated media are used for a wide variety of applications. To minimise the risks associated with powdered culture media, the majority of Merck's dehydrated culture media is produced in granular form. Merck uses ingredients of non animal origin to minimise the potential risk of transmissible spongiform encephalitis (TSE). When there is no alternative, only ingredients which are recommended by EDQM, and belonging to TSE category B or C are used. This ensures that the product is of the highest quality and in compliance with the standards for food control, microbiological testing on finished products and starting materials, and quality control in the pharmaceuticals and cosmetics industries. Custom-made media can be prepared to meet individual customer specifications. The formulation is kept confidential via a Non Disclosure Agreement. Please enquire for further details. Advantages of granulated Merck media.

Catalog Number: (3076.0100)
Supplier: VWR Chemicals
Description: Malachite green oxalate for microscopy (C.I. 42000)
UOM: 1 * 100 g

Catalog Number: (E772-25G)
Supplier: VWR Chemicals
Description: Bluish-green dye that is soluble in alcohol. Can be used with safranin as a stain for plant tissue and for demonstrating nuclear histones.
UOM: 1 * 25 g

Supplier: VWR Chemicals
Description: Methyl red, AnalaR NORMAPUR® ACS, Reag. Ph. Eur. C.I. 13020
Supplier: Merck
Description: Methyl red sodium salt
Catalog Number: (BSENC-1655-100)
Supplier: Biosensis
Description: mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). The mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties (1-3). The resulting monomeric protein is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which is monomeric and has an excitation maximum at 587 nm and and emission maximum at 610 nm (4).
UOM: 1 * 100 µl


Catalog Number: (AATB1144)
Supplier: AAT BIOQUEST
Description: mFluor™ Green 620 dye is a unique dye that is well excited by the green laser at 532 nm to give red fluorescence.
UOM: 1 * 5 mg


Catalog Number: (0394-25G)
Supplier: VWR Chemicals
Description: A valuable plasma stain used in contrast to hematoxylin. Also a counterstain to safranin.
UOM: 1 * 25 g

Catalog Number: (861235-25G)
Supplier: SIGMA ALDRICH MICROSCOPY
Description: Methyl red hydrochloride in ACS Reagent grade water, Sigma-Aldrich®
UOM: 1 * 25 g

Supplier: SIGMA ALDRICH MICROSCOPY
Description: The release of phosphate or pyrophosphate in an enzymatic reaction can be detected by malachite green and is measured spectrometrically at 660 nm.

Catalog Number: (46396-250MG)
Supplier: Merck
Description: Malachite green oxalate, Supelco®
UOM: 1 * 250 mg


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
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Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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