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Catalog Number: (BE-408)
Supplier: G-Biosciences
Description: In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of four different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.
UOM: 1 * 1 KIT


Catalog Number: (EDVOS-47)
Supplier: EDVOTEK
Description: Investigate how agarose gel electrophoresis unlocks the color code used by food scientists to make colorful candies.
UOM: 1 * 1 KIT


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Catalog Number: (BE-314)
Supplier: G-Biosciences
Description: A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.
UOM: 1 * 1 KIT


Catalog Number: (EDVOS-68)
Supplier: EDVOTEK
Description: Infectious agents such as bacteria and viruses can spread rapidly through a population and cause widespready disease and death. In this experiment, students use coloured solutions to simulate the spreading of a disease in the classroom.
UOM: 1 * 1 items


Catalog Number: (BE-406)
Supplier: G-Biosciences
Description: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.
UOM: 1 * 1 KIT


Catalog Number: (EDVO223/AP08)
Supplier: EDVOTEK
Description: In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish <i>Aequorea victoria</i>. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.
UOM: 1 * 1 items


Catalog Number: (EDVO266)
Supplier: EDVOTEK
Description: Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods.
UOM: 1 * 1 KIT


Catalog Number: (BE-312)
Supplier: G-Biosciences
Description: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).
UOM: 1 * 1 KIT


Supplier: EDVOTEK
Description: The three most frequently used restriction enzymes are Eco RI, Bam HI and Hind III. Each enzyme catalyses cleavage at the defined base sequence. All enzymes are lyophilised and contain 1500 units. One unit is defined as the amount of enzyme required to digest 1,0 µg of lambda DNA in 60 minutes at 37 °C in a total reaction mixture of 50 µl.

Catalog Number: (EDVO303)
Supplier: EDVOTEK
Description: Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.
UOM: 1 * 1 KIT


Catalog Number: (EDVO166)
Supplier: EDVOTEK
Description: An infectious outbreak requires prompt and accurate identification of the biological agent. Often, early clinical symptoms are first identified in exposed individuals and then infectious agents are identified by lab tests. In this experiment, students transmit a simulated infectious agent (chemical dye) between classmates which is only visible under long UV light. The pattern of transmission and primary source is documented.
UOM: 1 * 1 items


Catalog Number: (EDVO316)
Supplier: EDVOTEK
Description: Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.
UOM: 1 * 1 KIT


Catalog Number: (EDVO141)
Supplier: EDVOTEK
Description: Cancer cells differ from normal cells by the combinations of proteins that are present on their surfaces. Antibodies against these proteins will specifically bind to cancer cells and not to normal cells. This allows early detection of cancer and potentially a way of delivering cancer therapies. In this simulation experiment the reaction of cancer cell markers and their corresponding antigens are demonstrated.
UOM: 1 * 1 items

SDS


Catalog Number: (EDVO957)
Supplier: EDVOTEK
Description: According to the World Health Organisation, between two and three million cases of skin cancer occur globally every year. Many of these cases are caused by preventable damage to DNA by UV light. In this experiment, students expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analysed by agarose gel electrophoresis to observe the damage.
UOM: 1 * 1 KIT


Catalog Number: (EDVOS-70)
Supplier: EDVOTEK
Description: The body defends itself from attack by infectious agents like bacteria and viruses by producing antibodies. Enzyme linked immunosorbent assays (ELISA) test for antibodies present in the blood, which indicate infection. In this experiment, students perform a simulated ELISA test to identify infected samples and compare them to control samples.
UOM: 1 * 1 items

SDS


Catalog Number: (BE-407)
Supplier: G-Biosciences
Description: This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.
UOM: 1 * 1 KIT


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