You Searched For: BIOWEST

Supplier: Biowest

Description: Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

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Supplier: Biowest

Description: Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

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Supplier: Biowest

Description: Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

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Catalog Number: (L0014-100)

Supplier: Biowest

Description: Glutamine penicillin streptamycin acts against gram-negative and gram-positive bacteria.

UOM: 1 * 100 mL

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Catalog Number: (X0550-100)

Supplier: Biowest

Description: L(+)-Glutamine 0.2 mol/l in aqueous solution

UOM: 1 * 100 mL

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Supplier: Biowest

Description: Colcemid arrests the division of cells in mitosis. It prevents the formation of the spindle apparatus responsible for cell division, thereby permitting an accumulation of metaphases.

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Supplier: Biowest

Description: Amphotericin B used in tissue culture to prevent growth of yeasts and fungi. It changes the function and integrity of eucaryotic cell membranes by forming complexes with sterols (cholesterol) hence having no effect on bacteria. Cause leakage of glucose.

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Catalog Number: (L0207-500)

Supplier: Biowest

Description: Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, <i>Drosophila melanogaster</i>. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

UOM: 1 * 500 mL

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Catalog Number: (L0950-100)

Supplier: Biowest

Description: Accustase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is a direct gentle replacement for trypsin solution. It can also be used on suspension cells to reduce their agglutination in order to ease counting.

UOM: 1 * 100 mL

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Supplier: Biowest

Description: These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells <i>in vitro</i>. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

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Catalog Number: (X0557-100)

Supplier: Biowest

Description: Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

UOM: 1 * 100 mL

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Supplier: Biowest

Description: In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

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Catalog Number: (L0630-100)

Supplier: Biowest

Description: Versene can be used instead of trypsin. It is a chelating agent that disperses the cells by cutting the cytoplasmic bridges between them. Versene has the advantage of being able to withstand autoclave temperature, which is a guarantee of sterility. In addition, it is less aggressive cells than trypsin, which is useful for studies on cell growth.

UOM: 1 * 100 mL

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Supplier: Biowest

Description: Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.

Supplier: Biowest

Description: Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.

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Supplier: Biowest

Description: The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

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