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Product Image-PIER78835

Nuclear and cytoplasmic extraction reagents, NE-PER

Supplier: Thermo Fisher Scientific
Description: The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

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Chromogenic endotoxin testing reagents, Pyrochrome®

Catalog Number: (CAPEC1500-5)
Supplier: Associates of Cape Cod
Description: Pyrochrome® sets the standard for quality and redefines the way chromogenic reagents should perform. Pyrochrome is a versatile quantitative reagent that is used to perform kinetic or endpoint assays. It is a sensitive and flexible reagent that can be used for testing in compliance with the USP, EP and JP bacterial endotoxins test chapter. Pyrochrome is offered with either Pyrochrome Buffer or for endotoxin-specific testing, with Glucashield® Buffer. Pyrochrome can be used with the new Pyros Kinetix® Flex tube reader at a 1:1 and economical 4:1 ratio.
UOM: 1 * 300 Tests
Product Image-PIER15405

Pierce™ Immunoassay Microplates, Streptavidin Coated, 384-Well

Supplier: Thermo Fisher Scientific
Description: Pierce™ streptavidin coated PS microplates are ideal for binding assays using biotinylated antibodies. High binding capacity (HBC) plates provide a wider detection range and better curve linearity for small biotinylated ligands, such as peptides and oligonucleotides, than the standard coated plates. Available in clear for colorimetric assays, white for chemiluminescent assays, and black for fluorescent assays.

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6-Aminocaproic acid, white powder

Supplier: MP Biomedicals
Description: ε-Amino-n-caproic acid is a plasmin inhibitor. It inhibits carboxypeptidase B. It promotes rapid dissociation of plasmin by inhibiting the activation of plasminogen. As an inhibitor of plasmin it is has been utilised in the clotting buffer for fibrinogen assays.

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Cyanine 3-NHS ester chloride fluorescent labeling reagent

Catalog Number: (25-9004-72)
Supplier: Cytiva
Description: These versatile fluorophores can tolerate a pH range of 3 - 10 for use in a variety of applications at biologically relevant pHs. The dyes are also DMSO tolerant and photostable to enable transfer from storage to assay without loss of performance. The aqueous solubility eliminates the need for organic solvents in the assay buffers. Fluors are thoroughly QC tested to ensure high levels of chromophore and reactive dye content.
UOM: 1 * 150 µG
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Yeast viability kit for staining of live/dead yeast cells with AO/PI, ViaStain®

Supplier: NEXCELOM BIOSCIENCE
Description: Determining yeast viability and concentration during biofuel and brewing production is essential for process optimisation and monitoring of alcohol production.

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Anti-ALB Mouse Monoclonal Antibody [clone: SABN1-1]

Catalog Number: (PRSI34-011)
Supplier: ProSci Inc.
Description: Bovine serum albumin (BSA) is an abundant plasma protein in cows that is important for maintaining osmotic pressure in blood plasma for proper distribution of body fluids between intravascular compartments and body tissues. BSA is a common buffer component for immunoglobulin type assays due to good solubility characteristics for water, Ca2+, Na+, K+, fatty acids, hormones and bilirubin. It is a suitable reagent for laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling.
UOM: 1 * 100 µG

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One step RT-PCR master mix kit

Catalog Number: (71978-3)
Supplier: Merck Millipore (Novagen)
Description: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.
UOM: 1 * 1 KIT
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RPMI 1640 Medium without L-Glutamine, L-Serine, Methionine, HEPES (Powder)

Catalog Number: (USBIR8999-16-10L)
Supplier: US Biological
Description: RPMI-1640 was developed by Moore, <i>et. al</i>. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.
UOM: 1 * 1 items
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Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9%, white crystalline powder, Ultrapure

Supplier: MP Biomedicals
Description: Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is RNAse and DNAse-free. Tris is relatively non-hygroscopic ; but, if needed, it can be dried at 100 °C for up to 4 hours to remove any water.
Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions.

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Immunoassay microplates

Supplier: Thermo Fisher Scientific
Description: Designed specifically for immunoassay applications, this range of products have binding surfaces that include Immulon®, universal binding (UB) and enhanced binding (EB). These make good experimental design easier, allowing development of assays with stringent pH, buffer, pKa and resin requirements.

Environmentally Preferable

Product Image-735-0331

Microplates, 96-well, Nunc™ Edge 2.0

Supplier: Thermo Fisher Scientific
Description: The Nunc™ Edge plate has a design that minimises evaporation and the risks associated with the edge effect. When filled with sterile water or media, the surrounding moat acts as an evaporation buffer during extended incubation, yielding consistent, viable cells across the entire 96-well plate that in turn enhances productivity for cell-based assays. Enabling the use of all 96 wells allows researchers to plan experiments more efficiently, resulting in potentially less cost and related product waste.

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Anti-ALB Mouse Monoclonal Antibody [clone: ALB/398]

Catalog Number: (PRSI33-516)
Supplier: ProSci Inc.
Description: Reacts with a protein of ~66 kDa, identified as bovine serum albumin (BSA). It is a high affinity antibody and can be used for detection of traces of BSA. Bovine serum albumin (BSA) is an abundant plasma protein in cows that is important for maintaining osmotic pressure in blood plasma for proper distribution of body fluids between intravascular compartments and body tissues. BSA is a common buffer component for immunoglobulin type assays due to good solubility characteristics for water, Ca2+, Na+, K+, fatty acids, hormones and bilirubin. BSA makes up about half of the protein in plasma and represents the most stable and soluble protein in the plasma. It is a suitable reagent for laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling. The BSA component contains several lysines that are capable of reacting with conjugation sites of linkers, making it applicable as a carrier protein for antigenic compounds.
UOM: 1 * 100 µG

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ABsolute™ qPCR Low ROX Mixes

Supplier: Thermo Fisher Scientific
Description: ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.

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DNA gel extraction kit

Supplier: Biotium
Description: The DNA Gel Extraction Kit is a silica-gel, spin column based DNA extraction kit designed to purify 40 bp to 40 kb DNA fragments from agarose gels in TAE or TBE buffer.

SDS

Product Image-J373-4L

VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

Catalog Number: (J373-4L)
Supplier: VWR Chemicals
Description: Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
UOM: 1 * 4 L

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
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Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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