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Product Image-EK0031

T4 polynucleotide kinase

Supplier: Thermo Fisher Scientific
Description: Catalyses the transfer of the γ-phosphate from ATP to the 5'-hydroxyl group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction).

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Product Image-SM1103

DNA ladders, ultra low range, FastRuler

Catalog Number: (SM1103)
Supplier: Thermo Fisher Scientific
Description: FastRuler ultra low range DNA ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.
UOM: 1 * 1 KIT
Product Image-ER1751

Restriction enzymes, PfoI, Fermentas

Catalog Number: (ER1751)
Supplier: Thermo Fisher Scientific
Description: CCNGGA sites and cuts best at 37 °C in Tango buffer.
UOM: 1 * 1 KIT
Product Image-EP0742

Reverse transcriptase, Maxima™

Supplier: Thermo Fisher Scientific
Description: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Product Image-SM1821

RNA ladder, high range, RiboRuler™

Catalog Number: (SM1821)
Supplier: Thermo Fisher Scientific
Description: RiboRuler™ high range RNA ladder allows precise sizing of RNA molecules in denaturing agarose gels and Northern blotting.
UOM: 1 * 1 KIT
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Whole blood genomic DNA purification mini kit, GeneJET™

Supplier: Thermo Fisher Scientific
Description: GeneJET™ Whole Blood Genomic DNA Purification Mini Kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

Product Image-K0802

Plant RNA purification Mini kit, GeneJET™

Supplier: Thermo Fisher Scientific
Description: GeneJET™ Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from a wide variety of plant species and tissue types.

Product Image-K0241

Maxima™ SYBR green/fluorescein QPCR master mix (2X)

Supplier: Thermo Fisher Scientific
Description: The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

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DNA methylation analysis kit (MspI/HpaII), EpiJET™

Catalog Number: (K1441)
Supplier: Thermo Fisher Scientific
Description: 5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.
UOM: 1 * 1 KIT
Product Image-K0692

Gel extraction kit, GeneJET

Supplier: Thermo Fisher Scientific
Description: GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

Product Image-R0192

dNTP Mix, 10 mM

Supplier: Thermo Fisher Scientific
Description: dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

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Adenosine-5’-Triphosphate (ATP) 100 mM in aqueous solution ≥99% for molecular biology

Catalog Number: (R0441)
Supplier: Thermo Fisher Scientific
Description: ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. This nucleotide is used in different molecular biology applications, and it has greater than 99% purity.
UOM: 1 * 1 KIT

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Product Image-M1682

Maxima™ H minus cDNA synthesis master mix, with dsDNase

Supplier: Thermo Fisher Scientific
Description: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Product Image-ER0581

Restriction enzymes, NdeI, Fermentas

Supplier: Thermo Fisher Scientific
Description: TATG sites and cuts at 37 °C in O buffer (Isoschizomers: FauNDI).

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Product Image-ER0501

Restriction enzymes, HindIII, 10 U/μl, Fermentas

Supplier: Thermo Fisher Scientific
Description: HindIII restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions.

Product Image-ER0421

Restriction enzymes, Restriction enzyme, Eco147I (StuI), Fermentas

Catalog Number: (ER0421)
Supplier: Thermo Fisher Scientific
Description: CCT sites and cuts best at 37 °C in B buffer (Isoschizomers: AatI, PceI, SseBI, StuI).
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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