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qScript™ XLT cDNA SuperMix

Supplier: Quantabio
Description: qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).

SDS

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AccuStart™ II PCR SuperMix

Supplier: Quantabio
Description: AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

SDS Certificates

Product Image-786-12X

Protein quantitation assay kits, CB-X™ Protein assay

Supplier: G-Biosciences
Description: CB-X™ Protein Assay is a Coomassie dye based protein assay based on the Bradford Protein Assay. For protein samples in simple, uncomplicated aqueous buffers CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. For complicated protein samples, CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation in a single step. These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, buffering agents and chelating agents. The clean up stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay.

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AccuStart™ II GelTrack PCR SuperMix

Supplier: Quantabio
Description: AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II <i>Taq</i> DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

SDS Certificates

Product Image-521-2719

Microcentrifuge, 5420 (IVD marked)

Supplier: EPPENDORF
Description: Air-cooled 24-place microcentrifuge for everyday laboratory work. The range of methods includes DNA/ RNA purification, DNA extraction from agarose gels and PCR reaction set-up. The 5420 has a particularly small footprint to save space on the laboratory bench and an easy to clean keypad control panel.

Environmentally Preferable

Product Image-521-2717

Microcentrifuge, 5420 (General Laboratory Product)

Supplier: EPPENDORF
Description: Air-cooled 24-place microcentrifuge for everyday laboratory work. The range of methods includes DNA/ RNA purification, DNA extraction from agarose gels and PCR reaction set-up. The 5420 has a particularly small footprint to save space on the laboratory bench and an easy to clean keypad control panel.

Environmentally Preferable

Product Image-732-5511

Filter plates, 96-well, Corning® FiltrEX™

Supplier: Corning
Description: FiltrEX™ filter plates meet the industry standards for plate dimensions. The rigid side walls make the plate ideal for automation and the wide skirt accepts barcodes. Individual filter discs are encapsulated in the plate by a process that ensures 100% integrity of each well. The design of the nozzle prevents sample wicking and cross-contamination. Glass fibre filter plates can be used for a variety of applications, such as plasmid isolation, DNA purification, PCR clean-up or receptor/ligand binding assays. The low-binding hydrophilic PVDF membrane can be used for lysate clarification, protein kinase assays, or bead- or resin-based separation assays.

Certificates Environmentally Preferable

Product Image-80-6484-70

SDS-PAGE Clean Up Kit

Catalog Number: (80-6484-70)
Supplier: Cytiva
Description: SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.
UOM: 1 * 1 KIT
Product Image-BUNZ003000

Scouring hand pads

Catalog Number: (BUNZ003000)
Supplier: BUNZL
Description: These scouring pads are ideal for general washing up and mark removal.
UOM: 1 * 10 items
Product Image-80-6484-51

2D Clean-Up kit

Catalog Number: (80-6484-51)
Supplier: Cytiva
Description: The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.
UOM: 1 * 1 KIT

Certificates


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PCR master mix (2X)

Supplier: Thermo Fisher Scientific
Description: PCR Master Mix is a 2X concentrated solution of <i>Taq</i> DNA polymerase, dNTPs and all other components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimised for efficient and reproducible PCR.

Certificates

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DreamTaq™ PCR master mix (2X)

Supplier: Thermo Fisher Scientific
Description: DreamTaq™ PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq™ buffer, MgCl₂ and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. DreamTaq™ DNA polymerase is an enhanced <i>Taq</i> polymerase optimised for high throughput PCR applications. It ensures robust amplification of PCR products up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Product Image-710-1760

Real-Time PCR kit for detection of Legionella spec.

Supplier: Q BIOANALYTIC
Description: Legionella spec. is tested usually with cultural methods. However, this may take up to 14 days to deliver results and some strains are living but are not culturable. Using this Real-Time PCR test you may have results already after a few hours. You can use this test in combination with an appropriate pre- enrichment or you can do a DNA purification directly from the filter without pre-enrichment. The kit is also suitable to confirm bacterial colonies on agar plates.

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ScrubPADS UltraSOLV® Chamber Cleaning Pads

Supplier: FOAMTEC
Description: Hydrophilic urethane foam-based products work together for cleaning of recessed surfaces and microporous sub-surfaces. Safely and quickly remove build-up on process equipment and eliminate contamination. Use Silicon Carbide, Aluminium Oxide, or Diamond ScrubPADS for initial scouring. Ideal for aggressive cleaning, ScrubPADS, can be matched to the specific type of cleaning needed.

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First strand cDNA synthesis kit

Catalog Number: (K1612)
Supplier: Thermo Fisher Scientific
Description: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.
UOM: 1 * 1 KIT
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RevertAid™ First strand cDNA synthesis kit

Supplier: Thermo Fisher Scientific
Description: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
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This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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