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You Searched For: Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.


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TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit

Catalog Number: (ENZOENZ51006500)
Supplier: ENZO LIFE SCIENCES
Description: The kit contains two proprietary dyes suitable for visualizing nucleoli and nuclei simultaneously in living cells.
UOM: 1 * 1 KIT
Product Image-ENZOENZ51018K100

Membrane potential detection kit, MITO-ID®

Supplier: ENZO LIFE SCIENCES
Description: MITO-ID® membrane potential detection kit monitors energetic status using a simple mix-and-read, no-wash protocol. It measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status.

Product Image-ENZOENZ51026K500

ER-ID™ red assay kit (GFP-CERTIFIED®)

Catalog Number: (ENZOENZ51026K500)
Supplier: ENZO LIFE SCIENCES
Description: ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.
UOM: 1 * 1 KIT
Image Unavailable-ENZOALX850276KI01

Mitochondria/cytosol fractionation kit

Catalog Number: (ENZOALX850276KI01)
Supplier: ENZO LIFE SCIENCES
Description: For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.
UOM: 1 * 1 KIT
Image Unavailable-ENZOBMLAK5000001

FLUOR DE LYS® HDAC fluorometric activity assay kit

Catalog Number: (ENZOBMLAK5000001)
Supplier: ENZO LIFE SCIENCES
Description: The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.
UOM: 1 * 1 KIT
Image Unavailable-ENZOBMLAK5180001

FLUOR DE LYS® HDAC8 fluorometric drug discovery kit

Catalog Number: (ENZOBMLAK5180001)
Supplier: ENZO LIFE SCIENCES
Description: A Fluor-de-Lys® fluorescent assay system. The HDAC8 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC8 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC8 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.
UOM: 1 * 1 KIT
Image Unavailable-ENZOBMLAK5030001

FLUOR DE LYS® HDAC fluorometric cellular activity assay kit

Catalog Number: (ENZOBMLAK5030001)
Supplier: ENZO LIFE SCIENCES
Description: The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.
UOM: 1 * 1 KIT
Image Unavailable-ENZOADI9501500030

Blue IHC chromogen (AP), HIGHDEF®

Catalog Number: (ENZOADI9501500030)
Supplier: ENZO LIFE SCIENCES
Description: Blue IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or <i>in situ</i> hybridisation systems.
UOM: 1 * 30 mL
Image Unavailable-ENZOADI907029

Myeloperoxidase fluorometric detection kit

Catalog Number: (ENZOADI907029)
Supplier: ENZO LIFE SCIENCES
Description: The Myeloperoxidase fluorometric detection kit is a one-step no wash homogeneous activity assay that is adaptable to HTS applications. This kit offers highly sensitive fluorescent detection and the ability to monitor multiple time points to follow the kinetics of MPO activity.
UOM: 1 * 500 Tests
Image Unavailable-30025-1

Cell viability assay kits

Supplier: Biotium
Description: MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

SDS Certificates

Product Image-CAYM601130-1

Neutrophil/Monocyte Respiratory Burst Assay Kit

Catalog Number: (CAYM601130-1)
Supplier: Cayman Chemical
Description: Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.
UOM: 1 * 1 KIT
Product Image-30063.

TUNEL assay kits

Supplier: Biotium
Description: TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.

Image Unavailable-30072.

Apoptosis and necrosis assay kit, NucView™488 RedDot™2

Catalog Number: (30072.)
Supplier: Biotium
Description: This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.
UOM: 1 * 1 KIT
Product Image-30024.

Cytoplasmic membrane labelling kits, CellBrite™

Supplier: Biotium
Description: CellBrite kits are designed to label cell cytoplasmic membranes with a fluorescent membrane dye pre-dissolved as an optimised staining solution. By combining CellBrite membrane stains, it is possible to label multiple cell populations with distinctive colours, a capability useful for the studies of cell-cell interactions.

SDS

Product Image-CAYM11076-50

Epigenetics Screening Library

Catalog Number: (CAYM11076-50)
Supplier: Cayman Chemical
Description: For screening a variety of epigenetic compounds.
UOM: 1 * 50 µl
Product Image-CAYM10010721-1

S-Glutathionylated Protein Detection Kit

Catalog Number: (CAYM10010721-1)
Supplier: Cayman Chemical
Description: A convenient method for the direct visualisation of S-glutathionylated proteins in whole (permeabilised) cells.
UOM: 1 * 1 items
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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