Alcian blue 8GX, powder, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: C.I. 74240C.N. AnAn-6Ingrain Blue 1Alcian blue 8 GX

M-Clarity™ quality level = MQ100

A5268-25GEA 453 GBP
A5268-25G A5268-10G A5268-100G
Alcian blue 8GX, powder, Sigma-Aldrich®
Alcian blue 8 GX

Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.


  • Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices
  • It has been employed in the in vivo visualization of intravascular Bonghan ducts and corpuscles in murine models
  • It has been used to develop a novel histochemical staining method to assess multiple respiratory parameters in various species
  • Desired staining patterns can be obtained by manipulating pH and salt concentrations


The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. In the tissue, acid mucosubstances are stained selectively and nuclear fast red is commonly used as counterstain. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids can be visualized in addition. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1.

Formula: C₅₆H₆₈Cl₄CuN₁₆S₄
MW: 1298.86 g/mol
Storage Temperature: Room Temperature
MDL Number: MFCD00010720
CAS Number: 33864-99-2
EINECS: 251-705-7
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Specification Test Results

Appearance (Colour) Dark Blue to Very Dark Blue
Appearance (Form) Powder
Wavelength 615 - 630 nm
E1%
615-630 nm in H₂O
≥190
Dye Content
UV Spectroscopy, Based on E1% = 425 at 615-630 nm
45 - 65 %
Solubility (Colour) 1 mg/ml, H₂O Dark Blue to Very Dark Blue and Dark Blue-Green to Very Dark Blue-Green

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