Phusion® Hot Start II high-fidelity DNA polymerase

Supplier: FINNZYMES REAGENTS
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Phusion® Hot Start II high-fidelity DNA polymerase
Nucleic Acid Reagents End-point PCR Enzymes and Kits

Phusion® Hot Start II High-Fidelity DNA Polymerase allows the use of a wide range of primers, including those with low melting temperatures. Phusion® Hot Start II High-Fidelity DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications, such as cloning and high throughput PCR. It incorporates a unique dsDNA-binding domain making this Pyrococcus-like proofreading DNA polymerase extremely robust, rapid, and accurate.


  • No annealing temperature limitations
  • Allows reaction set up at room temperature
  • Zero-time reactivation due to unique Hot Start technology
  • Fast PCR enzyme with extreme fidelity (over 6 times more accurate than Pfu)
  • Robust enzyme with high tolerance against PCR inhibitors requires minimal optimisation

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