Taq DNA polymerase (recombinant)
Supplier: Thermo Fisher ScientificTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.
- Thermostable with half life more than 40 minutes at 95 °C
- Generates PCR products with 3'-dA overhangs
- Supplied with two buffers, including 10X Taq buffer with (NH₄)₂SO₄, which allows PCR at a wide range of magnesium concentrations and decreases unspecific priming
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labelled nucleotides)
Delivery information: Supplied with 10X Taq buffer with KCL, 10X Taq buffer with (NH₄)₂SO₄, and 25 mM MgCl₂.
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