3,3'-Diaminobenzidine tetrahydrochloride hydrate ≥96%, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPYTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
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Danger
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Synonyms:
DAB tetrahydrochloride hydrate
, DAB HCl hydrate
, 3,3',4,4'-Biphenyltetramine tetrahydrochloride hydrate
M-Clarity™ quality level = MQ200
3,3'-Diaminobenzidine tetrahydrochloride hydrate is a chromogen commonly used in immunohistochemistry and other staining techniques to visualise the presence of antigens or antibodies in biological samples. DAB HCl is designed as an immunohistology stain to visualise peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.
3,3′-Diaminobenzidine tetrahydrochloride hydrate is suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).
In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.
Formula:
C₁₂H₁₄N₄·4HCl·H₂O MW: 360.11 g/mol (anhy) Melting Pt: 280 °C Storage Temperature: Freezer |
MDL Number:
MFCD08273058 CAS Number: 868272-85-9 EINECS: 231-018-9 |
Specification Test Results
Appearance (Colour) | White to Light Brown and Faint Red and Faint Grey to Light Grey |
Appearance (Form) | Powder or Crystal or Chunk(s) |
Infrared Spectrum | Conforms to Structure |
Water (by Karl Fischer) | 7 - 12 % |
Titration with NaOH Specification is "Dried Basis" |
96.0 - 104.0 % |
Solubility (Turbidity) 1%, H2O |
Clear to Slightly Hazy |
Solubility (Colour) | Colorless to Faint Yellow and Very Faint Brown-Yellow to Light Brown-Yellow and Very Faint Red to Faint Red |
Recommended Retest Period | 3 Years |
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