3,3'-Diaminobenzidine tetrahydrochloride hydrate ≥96%, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: DAB tetrahydrochloride hydrateDAB HCl hydrate3,3',4,4'-Biphenyltetramine tetrahydrochloride hydrate

M-Clarity™ quality level = MQ200

D5637-1GEA 41 GBP
D5637-1G D5637-50G D5637-10G D5637-5G D5637-25G
3,3'-Diaminobenzidine tetrahydrochloride hydrate ≥96%, Sigma-Aldrich®
3,3'-Diaminobenzidine tetrahydrochloride hydrate

3,3'-Diaminobenzidine tetrahydrochloride hydrate is a chromogen commonly used in immunohistochemistry and other staining techniques to visualise the presence of antigens or antibodies in biological samples. DAB HCl is designed as an immunohistology stain to visualise peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.


3,3′-Diaminobenzidine tetrahydrochloride hydrate is suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).


In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.

Formula: C₁₂H₁₄N₄·4HCl·H₂O
MW: 360.11 g/mol (anhy)
Melting Pt: 280 °C
Storage Temperature: Freezer
MDL Number: MFCD08273058
CAS Number: 868272-85-9
EINECS: 231-018-9
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Specification Test Results

Appearance (Colour) White to Light Brown and Faint Red and Faint Grey to Light Grey
Appearance (Form) Powder or Crystal or Chunk(s)
Infrared Spectrum Conforms to Structure
Water (by Karl Fischer) 7 - 12 %
Titration with NaOH
Specification is "Dried Basis"
96.0 - 104.0 %
Solubility (Turbidity)
1%, H2O
Clear to Slightly Hazy
Solubility (Colour) Colorless to Faint Yellow and Very Faint Brown-Yellow to Light Brown-Yellow and Very Faint Red to Faint Red
Recommended Retest Period 3 Years

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