Acridine orange base ≥75% (dye content), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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M-Clarity™ quality level = MQ200

235474-25G 235474-5G
235474-25GEA 244 GBP
235474-25G 235474-5G
Acridine orange base ≥75% (dye content), Sigma-Aldrich®
Acridine orange

Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye. Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

Formula: C₁₇H₁₉N₃
Melting Pt: 165 °C (dec., lit.)
Storage Temperature: Ambient
MDL Number: MFCD00005029
CAS Number: 494-38-2
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Specification Test Results

Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Wavelength 227 - 233 nm
Extinction Coefficient ≥14000
Carbon ≥56.2 %
Nitrogen ≥11.6 %
Purity (HPLC) ≥75 %
Solubility (Turbidity) Clear
Solubility (Color); Orange to Very Dark Red-Brown, c = 1mg/ml, 1N HCl Conforms to Requirements

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